Preparation of an affinity cryogel column for lysozyme purification
Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design 22 was used to optimize the immobilization procedure of the T...
| Autores: | ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2017 |
| País: | Brasil |
| Institución: | UNESP |
| Repositorio: | Repositório Institucional da UNESP |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/169760 |
| Acceso en línea: | http://dx.doi.org/10.1080/01496395.2017.1318921 http://hdl.handle.net/11449/169760 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Adsorption affinity chromatography cryogel lysozyme Tris |
| Sumario: | Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design 22 was used to optimize the immobilization procedure of the Tris on the cryogel. A maximum adsorption for LYZ (149.5 mg/g) was achieved when 376 mg/mL of Tris and 3.06 mol/L of sodium borohydride were used during the Tris immobilization. Chromatographic separation of LYZ from chicken egg white was done with a purity of 92.13%. Results showed that the affinity cryogel was a potential separation medium for LYZ purification. |
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