Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method
The hermit crab Clibanarius vittatus is a typical organism from intertidal regions being considered as a good bioindicator of tributyltin presence at these environments. Thus this study presents the analytical performance and validation method for TBT quantification in tissues of C. vittatus by gas...
| Autores: | ; ; ; ; ; ; |
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| Tipo de documento: | Artigo |
| Estado: | Published version |
| Data de publicação: | 2012 |
| País: | Brasil |
| Recursos: | UNESP |
| Repositório: | Repositório Institucional da UNESP |
| Idioma: | Inglês |
| OAI Identifier: | oai:repositorio.unesp.br:11449/21164 |
| Acesso em linha: | http://dx.doi.org/10.1590/S0103-50532012000100007 http://hdl.handle.net/11449/21164 |
| Nível de acesso: | Acesso aberto |
| Palavra-chave: | crustaceans organotin tributyltin (TBT) gas chromatography |
| _version_ | 1878760866355085312 |
|---|---|
| author | Cristale, Joyce [UNESP] |
| author2 | dos Santos, Dayana M. Sant'Anna, Bruno S. [UNESP] Sandron, Daniela C. [UNESP] Cardoso, Sara [UNESP] Turra, Alexander Marchi, Mary Rosa Rodrigues de [UNESP] |
| author2_role | author author author author author author |
| author_browse | Cardoso, Sara [UNESP] Cristale, Joyce [UNESP] Marchi, Mary Rosa Rodrigues de [UNESP] Sandron, Daniela C. [UNESP] Sant'Anna, Bruno S. [UNESP] Turra, Alexander dos Santos, Dayana M. |
| author_facet | Cristale, Joyce [UNESP] dos Santos, Dayana M. Sant'Anna, Bruno S. [UNESP] Sandron, Daniela C. [UNESP] Cardoso, Sara [UNESP] Turra, Alexander Marchi, Mary Rosa Rodrigues de [UNESP] |
| author_role | author |
| collection | Repositório Institucional da UNESP |
| description | The hermit crab Clibanarius vittatus is a typical organism from intertidal regions being considered as a good bioindicator of tributyltin presence at these environments. Thus this study presents the analytical performance and validation method for TBT quantification in tissues of C. vittatus by gas chromatography with pulsed flame photometric detector (GC-PFPD) after extraction with an apolar solvent (toluene) and Grignard derivatization. The limits of detection of the method (LOD) were 2.0 and 2.8 ng g(-1) for TBT and DBT (dibutyltin), respectively, and its limits of quantification (LOQ) were 6.6 and 8.9 ng g(-1) for TBT and DBT, respectively. The method was applied to samples from Santos Estuary, São Paulo State, Brazil. TBT and DBT concentrations ranged from 26.7 to 175.0 ng g(-1) and from 46.2 to 156.0 ng g(-1), respectively. These concentrations are worrisome since toxic effects (such as endocrine disruption) have been reported for other organisms even under lower levels of registred at this study. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_9c8158a23b9b2e96bdca5c9cf5cc1549 |
| institution | UNESP |
| instname_str | Universidade Estadual Paulista (UNESP) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:repositorio.unesp.br:11449/21164 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | Soc Brasileira Quimica |
| publisherStr | Soc Brasileira Quimica |
| reponame_str | Repositório Institucional da UNESP |
| spelling | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method Cristale, Joyce [UNESP] dos Santos, Dayana M. Sant'Anna, Bruno S. [UNESP] Sandron, Daniela C. [UNESP] Cardoso, Sara [UNESP] Turra, Alexander Marchi, Mary Rosa Rodrigues de [UNESP] crustaceans organotin tributyltin (TBT) gas chromatography The hermit crab Clibanarius vittatus is a typical organism from intertidal regions being considered as a good bioindicator of tributyltin presence at these environments. Thus this study presents the analytical performance and validation method for TBT quantification in tissues of C. vittatus by gas chromatography with pulsed flame photometric detector (GC-PFPD) after extraction with an apolar solvent (toluene) and Grignard derivatization. The limits of detection of the method (LOD) were 2.0 and 2.8 ng g(-1) for TBT and DBT (dibutyltin), respectively, and its limits of quantification (LOQ) were 6.6 and 8.9 ng g(-1) for TBT and DBT, respectively. The method was applied to samples from Santos Estuary, São Paulo State, Brazil. TBT and DBT concentrations ranged from 26.7 to 175.0 ng g(-1) and from 46.2 to 156.0 ng g(-1), respectively. These concentrations are worrisome since toxic effects (such as endocrine disruption) have been reported for other organisms even under lower levels of registred at this study. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Apoio à Ciência Tecnologia e Educação (FACTE) São Paulo State Univ UNESP, Dept Analyt Chem, Inst Chem, BR-14800900 Araraquara, SP, Brazil Univ São Paulo, Dept Chem & Geol Oceanog, Oceanog Inst, BR-05508900 São Paulo, Brazil São Paulo State Univ UNESP, Dept Zool, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil Univ São Paulo, Dept Biol Oceanog, Oceanog Inst, BR-05508120 São Paulo, Brazil FAPESP: 06/57007-3 FAPESP: 06/61589-8 CNPq: 301240/2006-0 Soc Brasileira Quimica http://dx.doi.org/10.1590/S0103-50532012000100007 Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 23, n. 1, p. 39-45, 2012. 0103-5053 http://hdl.handle.net/11449/21164 10.1590/S0103-50532012000100007 S0103-50532012000100007 WOS:000300061300008 S0103-50532012000100007.pdf 2543372149131902 |
| spellingShingle | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method Cristale, Joyce [UNESP] crustaceans organotin tributyltin (TBT) gas chromatography |
| status_str | publishedVersion |
| title | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method |
| title_full | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method |
| title_fullStr | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method |
| title_full_unstemmed | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method |
| title_short | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method |
| title_sort | Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method |
| topic | crustaceans organotin tributyltin (TBT) gas chromatography |
| topic_facet | crustaceans organotin tributyltin (TBT) gas chromatography |
| url | http://dx.doi.org/10.1590/S0103-50532012000100007 http://hdl.handle.net/11449/21164 |