Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using...
| Authors: | ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2022 |
| Country: | Brasil |
| Institution: | UFC |
| Repository: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
| Language: | English |
| OAI Identifier: | oai:repositorio.ufc.br:riufc/81250 |
| Online Access: | http://repositorio.ufc.br/handle/riufc/81250 |
| Access Level: | Open access |
| Keyword: | Aquicultura Antibiótico Integron Aquaculture Antibiotic |
| _version_ | 1878757246409637888 |
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| author | Carvalho, Edirsana Maria Ribeiro |
| author2 | Menezes, Francisca Gleire Rodrigues de Santos, Daniel Rodrigues dos Souza, Oscarina Viana de Hofer, Ernesto Vieira, Regine Helena silva dos Fernandes |
| author2_role | author author author author author |
| author_browse | Carvalho, Edirsana Maria Ribeiro Hofer, Ernesto Menezes, Francisca Gleire Rodrigues de Santos, Daniel Rodrigues dos Souza, Oscarina Viana de Vieira, Regine Helena silva dos Fernandes |
| author_facet | Carvalho, Edirsana Maria Ribeiro Menezes, Francisca Gleire Rodrigues de Santos, Daniel Rodrigues dos Souza, Oscarina Viana de Hofer, Ernesto Vieira, Regine Helena silva dos Fernandes |
| author_role | author |
| bitstream_url_str_mv | http://repositorio.ufc.br/bitstream/riufc/81250/1/2022_art_emrcarvalho.pdf http://repositorio.ufc.br/bitstream/riufc/81250/2/license.txt |
| collection | Repositório Institucional da Universidade Federal do Ceará (UFC) |
| description | The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using the primers In, Int1, qacE∆1, sul1 and blaP1. Eight (32%) isolates yielded integrase (intI1) in chromosomal DNA belonging to class 1 integrons, with the gene cassette blaP1 detected in strains of V. natriengens (V10), V. coralliilyticus (V11) and V. parahaemolyticus (V25). The percentage was 100% in plasmids, with the detection of the gene cassettes blaP1 and ereA2 in V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) and V. mimicus (V22). The detection of antimicrobial resistance gene cassettes in the isolated strains indicates the possibility of dissemination of these genetic elements to autochthonous bacteria in shrimp culture environment. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_6c1015d20249df5b3f75bb535bb75ad2 |
| identifier_str_mv | 2236-6717 |
| institution | UFC |
| instname_str | Universidade Federal do Ceará (UFC) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:repositorio.ufc.br:riufc/81250 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | Revista Científica Semana Acadêmica |
| publisherStr | Revista Científica Semana Acadêmica |
| reponame_str | Repositório Institucional da Universidade Federal do Ceará (UFC) |
| spelling | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). Carvalho, Edirsana Maria Ribeiro Menezes, Francisca Gleire Rodrigues de Santos, Daniel Rodrigues dos Souza, Oscarina Viana de Hofer, Ernesto Vieira, Regine Helena silva dos Fernandes Aquicultura Antibiótico Integron Aquaculture Antibiotic The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using the primers In, Int1, qacE∆1, sul1 and blaP1. Eight (32%) isolates yielded integrase (intI1) in chromosomal DNA belonging to class 1 integrons, with the gene cassette blaP1 detected in strains of V. natriengens (V10), V. coralliilyticus (V11) and V. parahaemolyticus (V25). The percentage was 100% in plasmids, with the detection of the gene cassettes blaP1 and ereA2 in V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) and V. mimicus (V22). The detection of antimicrobial resistance gene cassettes in the isolated strains indicates the possibility of dissemination of these genetic elements to autochthonous bacteria in shrimp culture environment. O objetivo do presente estudo foi identificar integrons e cassetes gênicos em vibrios isolados da hemolinfa de camarão (Litopenaeus vannamei) cultivado no Ceará (Nordeste do Brasil). Vinte e cinco linhagens de vibrio foram avaliadas em PCR para integrons classe 1 em DNA cromossômico e plasmidial, utilizando os primers In, Int1, qacE∆1, sul1 e blaP1. Oito (32%) isolados produziram integrase (intI1) em DNA cromossômico pertencente a integrons classe 1, com o gene cassete blaP1 detectado em cepas de V. natriengens (V10), V. coralliilyticus (V11) e V. parahaemolyticus (V25). A porcentagem foi de 100% em plasmídeos, com a detecção dos cassetes gênicos blaP1 e ereA2 em V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) e V. mimicus (V22). A detecção de cassetes de genes de resistência antimicrobiana nas cepas isoladas indica a possibilidade de disseminação desses elementos genéticos para bactérias autóctones em ambiente de cultivo de camarão. Revista Científica Semana Acadêmica CARVALHO, Edirsana Maria Ribeiro; MENEZES, Francisca Gleire Rodrigues des; SANTOS, Daniel Rodrigues dos; SOUZA, Oscarina Viana de; HOFER, Ernesto; VIEIRA, Regine Helena Silva dos Fernandes. Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). Revista Científica Semana Acadêmica, v. 10, p. 1-16, 2022. Disponível em: https://semanaacademica.org.br/artigo/integrons-and-gene-cassettes-encoding-antimicrobial-resistance-vibrios-isolated-shrimp. Acesso em: 11 jun 2025. http://repositorio.ufc.br/handle/riufc/81250 2236-6717 |
| spellingShingle | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). Carvalho, Edirsana Maria Ribeiro Aquicultura Antibiótico Integron Aquaculture Antibiotic |
| status_str | publishedVersion |
| title | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). |
| title_full | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). |
| title_fullStr | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). |
| title_full_unstemmed | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). |
| title_short | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). |
| title_sort | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). |
| topic | Aquicultura Antibiótico Integron Aquaculture Antibiotic |
| topic_facet | Aquicultura Antibiótico Integron Aquaculture Antibiotic |
| url | http://repositorio.ufc.br/handle/riufc/81250 |