Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatogr...
| Autores: | ; ; |
|---|---|
| Tipo de documento: | Artigo |
| Estado: | Published version |
| Data de publicação: | 2002 |
| País: | Brasil |
| Recursos: | EMBRAPA |
| Repositório: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
| Idioma: | Inglês |
| OAI Identifier: | oai:www.alice.cnptia.embrapa.br:doc/15040 |
| Acesso em linha: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040 |
| Nível de acesso: | Acesso aberto |
| Palavra-chave: | Cromatografia Hymenaea Courbaril |
| Resumo: | Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS). |
|---|