Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
Fiocruz-CNPq
| Autores: | ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2006 |
| País: | Brasil |
| Institución: | FIOCRUZ |
| Repositorio: | Repositório Institucional da FIOCRUZ (ARCA) |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:arca.fiocruz.br:icict/6570 |
| Acceso en línea: | https://arca.fiocruz.br/handle/icict/6570 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Praziquantel Fluorescence Hoechst 33258 LysoTracker Red Lectin of Glycine max Sporocysts Schistosoma mansoni |
| _version_ | 1878753891550494720 |
|---|---|
| author | Mattos, Ana Carolina Alves de |
| author2 | Kusel, John Robert Pimenta, Paulo Filemon Paolucci Coelho, Paulo Marcos Zach |
| author2_role | author author author |
| author_browse | Coelho, Paulo Marcos Zach Kusel, John Robert Mattos, Ana Carolina Alves de Pimenta, Paulo Filemon Paolucci |
| author_facet | Mattos, Ana Carolina Alves de Kusel, John Robert Pimenta, Paulo Filemon Paolucci Coelho, Paulo Marcos Zach |
| author_role | author |
| bitstream_url_str_mv | https://arca.fiocruz.br/bitstreams/a3770429-311c-4f91-919c-76d3b9272da7/download https://arca.fiocruz.br/bitstreams/e4581674-f5a3-4b77-96b3-a5607b2c4d6b/download https://arca.fiocruz.br/bitstreams/7f1885cd-d562-4fb2-87dc-1cc8d389a713/download https://arca.fiocruz.br/bitstreams/1866a353-2a48-4ffd-9d47-6a0a78f4fd06/download |
| collection | Repositório Institucional da FIOCRUZ (ARCA) |
| description | Fiocruz-CNPq |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_3dd25f45cca2cdee115b2ca4e60e4b5a |
| identifier_str_mv | 10.1590/S0074-02762006000900044 |
| institution | FIOCRUZ |
| instname_str | Fundação Oswaldo Cruz (FIOCRUZ) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:arca.fiocruz.br:icict/6570 |
| publishDate | 2006 |
| publishDateSort | 2006 |
| reponame_str | Repositório Institucional da FIOCRUZ (ARCA) |
| spelling | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts Mattos, Ana Carolina Alves de Kusel, John Robert Pimenta, Paulo Filemon Paolucci Coelho, Paulo Marcos Zach Praziquantel Fluorescence Hoechst 33258 LysoTracker Red Lectin of Glycine max Sporocysts Schistosoma mansoni Fiocruz-CNPq Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. University of Glasgow. IBLS Division of Infection and Immunity. Scotland, UK. Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil. Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Santa Casa de Misericórdia de Belo Horizonte. Belo Horizonte, MG, Brasil. Praziquantel (PZQ) is effective against all the evolutive phases of Schistosoma mansoni. Infected Biomphalaria glabrata snails have their cercarial shedding interrupted when exposed to PZQ. Using primary in vitro transformed sporocysts, labeled with the probe Hoechst 33258 (indicator of membrane integrity), and lectin of Glycine max (specific for carbohydrate of N-acetylgalactosamine membrane), we evaluated the presence of lysosomes at this evolutive phase of S. mansoni, as well as the influence of PZQ on these acidic organelles and on the tegument of the sporocyst. Although the sporocyst remained alive, it was observed that there was a marked contraction of its musculature, and there occurred a change in the parasite's structure. Also, the acidic vesicles found in the sporo-cysts showed a larger delimited area after contact of the parasites with PZQ. Damages to the tegument was also observed, as show a well-marked labeling either with Hoechst 33258 or with lectin of Glycine max after contact of sporocysts with the drug. These results could partially explain the interruption/reduction mechanism of cercarial shedding in snails exposed to PZQ. MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006. 0074-0276 https://arca.fiocruz.br/handle/icict/6570 10.1590/S0074-02762006000900044 |
| spellingShingle | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts Mattos, Ana Carolina Alves de Praziquantel Fluorescence Hoechst 33258 LysoTracker Red Lectin of Glycine max Sporocysts Schistosoma mansoni |
| status_str | publishedVersion |
| title | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts |
| title_full | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts |
| title_fullStr | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts |
| title_full_unstemmed | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts |
| title_short | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts |
| title_sort | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts |
| topic | Praziquantel Fluorescence Hoechst 33258 LysoTracker Red Lectin of Glycine max Sporocysts Schistosoma mansoni |
| topic_facet | Praziquantel Fluorescence Hoechst 33258 LysoTracker Red Lectin of Glycine max Sporocysts Schistosoma mansoni |
| url | https://arca.fiocruz.br/handle/icict/6570 |