The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins
Plants deal with cold temperatures via different signal transduction pathways. The HD-Zip I homologous transcription factors HaHB1 from sunflower and AtHB13 from Arabidopsis were identified as playing a key role in such cold response. The expression patterns of both genes were analyzed indicating an...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2012 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/60486 |
| Online Access: | http://hdl.handle.net/11336/60486 |
| Access Level: | Open access |
| Keyword: | Abiotic Stress Antifreeze Proteins Athb13 Chitinase Freezing Tolerance Glucanase Hahb1 Hd-Zip Pathogenesis-Related Proteins https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878404057100451840 |
|---|---|
| author | Cabello, Julieta Virginia |
| author2 | Arce, Agustín Lucas Chan, Raquel Lia |
| author2_role | author author |
| author_browse | Arce, Agustín Lucas Cabello, Julieta Virginia Chan, Raquel Lia |
| author_facet | Cabello, Julieta Virginia Arce, Agustín Lucas Chan, Raquel Lia |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Plants deal with cold temperatures via different signal transduction pathways. The HD-Zip I homologous transcription factors HaHB1 from sunflower and AtHB13 from Arabidopsis were identified as playing a key role in such cold response. The expression patterns of both genes were analyzed indicating an up-regulation by low temperatures. When these genes were constitutively expressed in Arabidopsis, the transgenic plants showed similar phenotypes including cell membrane stabilization under freezing treatments and cold tolerance. An exploratory transcriptomic analysis of HaHB1 transgenic plants indicated that several transcripts encoding glucanases and chitinases were induced. Moreover, under freezing conditions some proteins accumulated in HaHB1 plants apoplasts and these extracts exerted antifreeze activity in vitro. Three genes encoding two glucanases and a chitinase were overexpressed in Arabidopsis and these plants were able to tolerate freezing temperatures. All the obtained transgenic plants exhibited cell membrane stabilization after a short freezing treatment. Finally, HaHB1 and AtHB13 were used to transiently transform sunflower and soybean leading to the up-regulation of HaHB1/AtHB13-target homologues thus indicating the conservation of cold response pathways. We propose that HaHB1 and AtHB13 are involved in plant cold tolerance via the induction of proteins able to stabilize cell membranes and inhibit ice growth. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_f3155d0e9d97f0a97d28fa92f9c05cb7 |
| identifier_str_mv | Cabello, Julieta Virginia; Arce, Agustín Lucas; Chan, Raquel Lia; The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins; Wiley Blackwell Publishing, Inc; Plant Journal; 69; 1; 1-2012; 141-153 0960-7412 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/60486 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley Blackwell Publishing, Inc |
| publisherStr | Wiley Blackwell Publishing, Inc |
| reponame_str | CONICET Digital (CONICET) |
| spelling | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins Cabello, Julieta Virginia Arce, Agustín Lucas Chan, Raquel Lia Abiotic Stress Antifreeze Proteins Athb13 Chitinase Freezing Tolerance Glucanase Hahb1 Hd-Zip Pathogenesis-Related Proteins https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Plants deal with cold temperatures via different signal transduction pathways. The HD-Zip I homologous transcription factors HaHB1 from sunflower and AtHB13 from Arabidopsis were identified as playing a key role in such cold response. The expression patterns of both genes were analyzed indicating an up-regulation by low temperatures. When these genes were constitutively expressed in Arabidopsis, the transgenic plants showed similar phenotypes including cell membrane stabilization under freezing treatments and cold tolerance. An exploratory transcriptomic analysis of HaHB1 transgenic plants indicated that several transcripts encoding glucanases and chitinases were induced. Moreover, under freezing conditions some proteins accumulated in HaHB1 plants apoplasts and these extracts exerted antifreeze activity in vitro. Three genes encoding two glucanases and a chitinase were overexpressed in Arabidopsis and these plants were able to tolerate freezing temperatures. All the obtained transgenic plants exhibited cell membrane stabilization after a short freezing treatment. Finally, HaHB1 and AtHB13 were used to transiently transform sunflower and soybean leading to the up-regulation of HaHB1/AtHB13-target homologues thus indicating the conservation of cold response pathways. We propose that HaHB1 and AtHB13 are involved in plant cold tolerance via the induction of proteins able to stabilize cell membranes and inhibit ice growth. Fil: Cabello, Julieta Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Wiley Blackwell Publishing, Inc http://hdl.handle.net/11336/60486 Cabello, Julieta Virginia; Arce, Agustín Lucas; Chan, Raquel Lia; The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins; Wiley Blackwell Publishing, Inc; Plant Journal; 69; 1; 1-2012; 141-153 0960-7412 CONICET Digital CONICET |
| spellingShingle | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins Cabello, Julieta Virginia Abiotic Stress Antifreeze Proteins Athb13 Chitinase Freezing Tolerance Glucanase Hahb1 Hd-Zip Pathogenesis-Related Proteins https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins |
| title_full | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins |
| title_fullStr | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins |
| title_full_unstemmed | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins |
| title_short | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins |
| title_sort | The homologous HD-Zip i transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins |
| topic | Abiotic Stress Antifreeze Proteins Athb13 Chitinase Freezing Tolerance Glucanase Hahb1 Hd-Zip Pathogenesis-Related Proteins https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | Abiotic Stress Antifreeze Proteins Athb13 Chitinase Freezing Tolerance Glucanase Hahb1 Hd-Zip Pathogenesis-Related Proteins https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/60486 |