Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity
Acinetobacter baumannii is a multidrug resistant nosocomial pathogen that shows an outstanding ability to undergo genetic exchange, thereby acquiring different traits that contribute to its success. In this work, we identified genetic features of an indigo-pigmented A. baumannii strain (Ab33405) tha...
| Authors: | ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2018 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/86950 |
| Online Access: | http://hdl.handle.net/11336/86950 |
| Access Level: | Open access |
| Keyword: | ACINETOBACTER BAUMANNII indigo pigmented extensively drug resistant genome analysis https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878404050174607360 |
|---|---|
| author | Traglia, German Matias |
| author2 | Chiem, Kevin Quinn, Brettni Fernandez, Jennifer S. Montaña, Sabrina Daiana Almuzara, Marisa Mussi, María Alejandra Tolmasky, Marcelo E. Iriarte, Andres Centron, Daniela Ramirez, Maria Soledad |
| author2_role | author author author author author author author author author author |
| author_browse | Almuzara, Marisa Centron, Daniela Chiem, Kevin Fernandez, Jennifer S. Iriarte, Andres Montaña, Sabrina Daiana Mussi, María Alejandra Quinn, Brettni Ramirez, Maria Soledad Tolmasky, Marcelo E. Traglia, German Matias |
| author_facet | Traglia, German Matias Chiem, Kevin Quinn, Brettni Fernandez, Jennifer S. Montaña, Sabrina Daiana Almuzara, Marisa Mussi, María Alejandra Tolmasky, Marcelo E. Iriarte, Andres Centron, Daniela Ramirez, Maria Soledad |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Acinetobacter baumannii is a multidrug resistant nosocomial pathogen that shows an outstanding ability to undergo genetic exchange, thereby acquiring different traits that contribute to its success. In this work, we identified genetic features of an indigo-pigmented A. baumannii strain (Ab33405) that belongs to the clonal complex CC113 B /CC79 P . Ab33405 possesses a high number of genes coding for antibiotic resistance and virulence factors that may contribute to its survival, not only in the human host, but also in the hospital environment. Thirteen genes conferring resistance to different antibiotic families (trimethoprim, florfenicol, β-lactams, aminoglycosides and sulfonamide) as well as the adeIJK genes and the capsule locus (KL) and outer core locus (OCL) were identified. Ab33405 includes 250 unique genes and a significant number of elements associated with Horizontal Gene Transfer, such as insertion sequences and transposons, genomic islands and prophage sequences. Also, the indigo-pigmented uncommon phenotype that could be associated with the monooxygenase or dioxygenase enzyme coded for by the iacA gene within the iac cluster was probably conferred by insertion of a 18-kb DNA fragment into the iacG gene belonging to this cluster. The Ab33405 genome includes all type VI secretion system genes and killing assays showed the ability of Ab33045 to kill Escherichia coli. In addition, Ab33405 can modulate susceptibility antibiotics when exposed to blue light. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_f21558d8122fbffc8a67ec8f5d52cf3f |
| identifier_str_mv | Traglia, German Matias; Chiem, Kevin; Quinn, Brettni; Fernandez, Jennifer S.; Montaña, Sabrina Daiana; et al.; Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity; Nature Publishing Group; Scientific Reports; 8; 16961; 12-2018; 1-15 2045-2322 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/86950 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | Nature Publishing Group |
| publisherStr | Nature Publishing Group |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity Traglia, German Matias Chiem, Kevin Quinn, Brettni Fernandez, Jennifer S. Montaña, Sabrina Daiana Almuzara, Marisa Mussi, María Alejandra Tolmasky, Marcelo E. Iriarte, Andres Centron, Daniela Ramirez, Maria Soledad ACINETOBACTER BAUMANNII indigo pigmented extensively drug resistant genome analysis https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Acinetobacter baumannii is a multidrug resistant nosocomial pathogen that shows an outstanding ability to undergo genetic exchange, thereby acquiring different traits that contribute to its success. In this work, we identified genetic features of an indigo-pigmented A. baumannii strain (Ab33405) that belongs to the clonal complex CC113 B /CC79 P . Ab33405 possesses a high number of genes coding for antibiotic resistance and virulence factors that may contribute to its survival, not only in the human host, but also in the hospital environment. Thirteen genes conferring resistance to different antibiotic families (trimethoprim, florfenicol, β-lactams, aminoglycosides and sulfonamide) as well as the adeIJK genes and the capsule locus (KL) and outer core locus (OCL) were identified. Ab33405 includes 250 unique genes and a significant number of elements associated with Horizontal Gene Transfer, such as insertion sequences and transposons, genomic islands and prophage sequences. Also, the indigo-pigmented uncommon phenotype that could be associated with the monooxygenase or dioxygenase enzyme coded for by the iacA gene within the iac cluster was probably conferred by insertion of a 18-kb DNA fragment into the iacG gene belonging to this cluster. The Ab33405 genome includes all type VI secretion system genes and killing assays showed the ability of Ab33045 to kill Escherichia coli. In addition, Ab33405 can modulate susceptibility antibiotics when exposed to blue light. Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Chiem, Kevin. California State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Quinn, Brettni. California State University; Estados Unidos Fil: Fernandez, Jennifer S.. California State University; Estados Unidos Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Almuzara, Marisa. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; Argentina Fil: Mussi, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos Fil: Iriarte, Andres. Universidad de la República; Uruguay Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Ramirez, Maria Soledad. California State University; Estados Unidos Nature Publishing Group http://hdl.handle.net/11336/86950 Traglia, German Matias; Chiem, Kevin; Quinn, Brettni; Fernandez, Jennifer S.; Montaña, Sabrina Daiana; et al.; Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity; Nature Publishing Group; Scientific Reports; 8; 16961; 12-2018; 1-15 2045-2322 CONICET Digital CONICET |
| spellingShingle | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity Traglia, German Matias ACINETOBACTER BAUMANNII indigo pigmented extensively drug resistant genome analysis https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity |
| title_full | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity |
| title_fullStr | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity |
| title_full_unstemmed | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity |
| title_short | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity |
| title_sort | Genome sequence analysis of an extensively drug-resistant Acinetobacter baumannii indigo-pigmented strain depicts evidence of increase genome plasticity |
| topic | ACINETOBACTER BAUMANNII indigo pigmented extensively drug resistant genome analysis https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | ACINETOBACTER BAUMANNII indigo pigmented extensively drug resistant genome analysis https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/86950 |