Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid
The modified Hodge method (MHT) has been recommended by the CLSI for confirmation of suspected class A carbapenemase production in species of Enterobacteriaceae. This test and the Masuda method (MAS) have advantages over traditional phenotypic methods in that they directly analyze carbapenemase acti...
| Authors: | ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2010 |
| Country: | Argentina |
| Institution: | ANLIS |
| Repository: | Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
| Language: | English |
| OAI Identifier: | oai:sgc.anlis.gob.ar:Publications/123456789/269 |
| Online Access: | http://jcm.asm.org/content/48/4/1323.full.pdf+html http://sgc.anlis.gob.ar/handle/123456789/269 |
| Access Level: | Open access |
| Keyword: | Enterobacteriaceae Reacciones Falso Positivas Humanos Ácidos Borónicos Oxacilina |
| _version_ | 1878403995041529856 |
|---|---|
| author | Pasteran, Fernando |
| author2 | Mendez, Tania Rapoport, Melina J. Guerriero, Leonor Corso, Alejandra |
| author2_role | author author author author |
| author_browse | Corso, Alejandra Guerriero, Leonor Mendez, Tania Pasteran, Fernando Rapoport, Melina J. |
| author_facet | Pasteran, Fernando Mendez, Tania Rapoport, Melina J. Guerriero, Leonor Corso, Alejandra |
| author_role | author |
| collection | Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
| description | The modified Hodge method (MHT) has been recommended by the CLSI for confirmation of suspected class A carbapenemase production in species of Enterobacteriaceae. This test and the Masuda method (MAS) have advantages over traditional phenotypic methods in that they directly analyze carbapenemase activity. In order to identify the potential interferences of these tests, we designed a panel composed of diverse bacterial genera with distinct carbapenem susceptibility patterns (42 carbapenemase producers and 48 nonproducers). About 25% of results among carbapenemase nonproducers, mainly strains harboring CTX-M and AmpC hyperproducers, were observed to be false positive. Subsequently, we developed an optimized approach for moreaccurate detection of suspicious isolates of carbapenemase by addition of boronic acid (BA) derivatives (reversible inhibitor of class A carbapenemases and AmpC cephalosporinases) and oxacillin (inhibitor of AmpCs enzymes). The use of the modified BA- and oxacillin-based MHT and MAS resulted in high sensitivity (>90%) and specificity (100%) for class A carbapenemase detection. By use of these methodologies, isolates producing KPCs and GES, Sme, IMI, and NMC-A carbapenemases were successfully distinguished from those producing other classes of ß-lactamases (extended-spectrum -lactamases [ESBLs], AmpC -lactamases, metallo- -lactamases [MBLs], etc.). These methods will provide the fast and useful information needed for targeting of antimicrobial therapy and appropriate infection control. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_e7870b10c00a2f0966ae46e2ab04a98f |
| identifier_str_mv | 1098-660X |
| institution | ANLIS |
| instname_str | Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:sgc.anlis.gob.ar:Publications/123456789/269 |
| publishDate | 2010 |
| publishDateSort | 2010 |
| reponame_str | Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
| spelling | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid Pasteran, Fernando Mendez, Tania Rapoport, Melina J. Guerriero, Leonor Corso, Alejandra Enterobacteriaceae Reacciones Falso Positivas Humanos Ácidos Borónicos Oxacilina The modified Hodge method (MHT) has been recommended by the CLSI for confirmation of suspected class A carbapenemase production in species of Enterobacteriaceae. This test and the Masuda method (MAS) have advantages over traditional phenotypic methods in that they directly analyze carbapenemase activity. In order to identify the potential interferences of these tests, we designed a panel composed of diverse bacterial genera with distinct carbapenem susceptibility patterns (42 carbapenemase producers and 48 nonproducers). About 25% of results among carbapenemase nonproducers, mainly strains harboring CTX-M and AmpC hyperproducers, were observed to be false positive. Subsequently, we developed an optimized approach for moreaccurate detection of suspicious isolates of carbapenemase by addition of boronic acid (BA) derivatives (reversible inhibitor of class A carbapenemases and AmpC cephalosporinases) and oxacillin (inhibitor of AmpCs enzymes). The use of the modified BA- and oxacillin-based MHT and MAS resulted in high sensitivity (>90%) and specificity (100%) for class A carbapenemase detection. By use of these methodologies, isolates producing KPCs and GES, Sme, IMI, and NMC-A carbapenemases were successfully distinguished from those producing other classes of ß-lactamases (extended-spectrum -lactamases [ESBLs], AmpC -lactamases, metallo- -lactamases [MBLs], etc.). These methods will provide the fast and useful information needed for targeting of antimicrobial therapy and appropriate infection control. Fil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina. Fil: Mendez, Tania. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina. Fil: Rapoport, Melina J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina. Fil: Guerriero, Leonor. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina. Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina. 1098-660X http://jcm.asm.org/content/48/4/1323.full.pdf+html http://sgc.anlis.gob.ar/handle/123456789/269 |
| spellingShingle | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid Pasteran, Fernando Enterobacteriaceae Reacciones Falso Positivas Humanos Ácidos Borónicos Oxacilina |
| status_str | publishedVersion |
| title | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid |
| title_full | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid |
| title_fullStr | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid |
| title_full_unstemmed | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid |
| title_short | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid |
| title_sort | Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid |
| topic | Enterobacteriaceae Reacciones Falso Positivas Humanos Ácidos Borónicos Oxacilina |
| topic_facet | Enterobacteriaceae Reacciones Falso Positivas Humanos Ácidos Borónicos Oxacilina |
| url | http://jcm.asm.org/content/48/4/1323.full.pdf+html http://sgc.anlis.gob.ar/handle/123456789/269 |