Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli
Objectives: Starting from the observation that Escherichia coli tolC mutations severely reduced the high-level resistance to tetracycline afforded by Tn10- and plasmid-encoded Tet(A) pumps, we studied the mechanism of this susceptibility. Methods: The MIC of tetracycline for MC4100 tolC::Tn10 and se...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2006 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/95302 |
| Online Access: | http://hdl.handle.net/11336/95302 |
| Access Level: | Open access |
| Keyword: | E. COLI TET(A)-ACRAB INTERPLAY TETRACYCLINE SUSCEPTIBILITY TOLC MUTATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403982586544128 |
|---|---|
| author | de Cristobal, Ricardo Ezequiel |
| author2 | Vincent, Paula Andrea Salomon, Raul Armando |
| author2_role | author author |
| author_browse | Salomon, Raul Armando Vincent, Paula Andrea de Cristobal, Ricardo Ezequiel |
| author_facet | de Cristobal, Ricardo Ezequiel Vincent, Paula Andrea Salomon, Raul Armando |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Objectives: Starting from the observation that Escherichia coli tolC mutations severely reduced the high-level resistance to tetracycline afforded by Tn10- and plasmid-encoded Tet(A) pumps, we studied the mechanism of this susceptibility. Methods: The MIC of tetracycline for MC4100 tolC::Tn10 and several tolC mutants carrying the Tn10 in other sites on the chromosome (thr::Tn10) was determined. The effect of a tolC mutation on the level of expression of Tn10 tet (A) was examined by using a tet (A)::lacZ gene fusion. Influence of tolC mutations on tetracycline efflux and accumulation was quantified by spectrofluorometric assays. The contribution of the AcrAB multidrug efflux system to high-level tetracycline resistance was measured in a Tn 10-carrying acrAB null mutant strain. Results: Tn10- and plasmid-encoded Tet(A) conferred 5- to 6-fold lower levels of tetracycline resistance in tolC mutants, as compared with control strain tolC+. Spectrofluorometric analyses showed that this resulted from a decrease in drug efflux in tolC mutants. Chlortetracycline resistance was also compromised by loss of TolC. Mutational loss of the AcrAB multidrug efflux transporter had the same effect as tolC mutations on tetracycline resistance. This indicated that tolC mutations act through inactivation of the AcrAB system. Conclusions: Our results are compatible with the hypothesis that the AcrAB pump is an important component in the development of high levels of resistance to tetracycline in E. coli, perhaps by working in combination with Tet(A). © 2006 Oxford University Press. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_e4d2e7dfdba62de42a1a115dec3ff0e5 |
| identifier_str_mv | de Cristobal, Ricardo Ezequiel; Vincent, Paula Andrea; Salomon, Raul Armando; Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli; Oxford University Press; Journal of Antimicrobial Chemotherapy; 58; 1; 7-2006; 31-36 0305-7453 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/95302 |
| publishDate | 2006 |
| publishDateSort | 2006 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli de Cristobal, Ricardo Ezequiel Vincent, Paula Andrea Salomon, Raul Armando E. COLI TET(A)-ACRAB INTERPLAY TETRACYCLINE SUSCEPTIBILITY TOLC MUTATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Objectives: Starting from the observation that Escherichia coli tolC mutations severely reduced the high-level resistance to tetracycline afforded by Tn10- and plasmid-encoded Tet(A) pumps, we studied the mechanism of this susceptibility. Methods: The MIC of tetracycline for MC4100 tolC::Tn10 and several tolC mutants carrying the Tn10 in other sites on the chromosome (thr::Tn10) was determined. The effect of a tolC mutation on the level of expression of Tn10 tet (A) was examined by using a tet (A)::lacZ gene fusion. Influence of tolC mutations on tetracycline efflux and accumulation was quantified by spectrofluorometric assays. The contribution of the AcrAB multidrug efflux system to high-level tetracycline resistance was measured in a Tn 10-carrying acrAB null mutant strain. Results: Tn10- and plasmid-encoded Tet(A) conferred 5- to 6-fold lower levels of tetracycline resistance in tolC mutants, as compared with control strain tolC+. Spectrofluorometric analyses showed that this resulted from a decrease in drug efflux in tolC mutants. Chlortetracycline resistance was also compromised by loss of TolC. Mutational loss of the AcrAB multidrug efflux transporter had the same effect as tolC mutations on tetracycline resistance. This indicated that tolC mutations act through inactivation of the AcrAB system. Conclusions: Our results are compatible with the hypothesis that the AcrAB pump is an important component in the development of high levels of resistance to tetracycline in E. coli, perhaps by working in combination with Tet(A). © 2006 Oxford University Press. Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; Argentina Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; Argentina Fil: Salomon, Raul Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; Argentina Oxford University Press http://hdl.handle.net/11336/95302 de Cristobal, Ricardo Ezequiel; Vincent, Paula Andrea; Salomon, Raul Armando; Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli; Oxford University Press; Journal of Antimicrobial Chemotherapy; 58; 1; 7-2006; 31-36 0305-7453 CONICET Digital CONICET |
| spellingShingle | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli de Cristobal, Ricardo Ezequiel E. COLI TET(A)-ACRAB INTERPLAY TETRACYCLINE SUSCEPTIBILITY TOLC MUTATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli |
| title_full | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli |
| title_fullStr | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli |
| title_full_unstemmed | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli |
| title_short | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli |
| title_sort | Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli |
| topic | E. COLI TET(A)-ACRAB INTERPLAY TETRACYCLINE SUSCEPTIBILITY TOLC MUTATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | E. COLI TET(A)-ACRAB INTERPLAY TETRACYCLINE SUSCEPTIBILITY TOLC MUTATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/95302 |