Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
A porphyrin-fullerene C60 dyad (TCP-C60) substituted by carbazoyl groups was used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers, holding fullerene units. The properties of this film were...
| Authors: | ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2015 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/180210 |
| Online Access: | http://hdl.handle.net/11336/180210 |
| Access Level: | Open access |
| Keyword: | POLYMERIC FILMS PORPHYRIN FULLERENE DYAD PHOTODYNAMIC INACTIVATION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| _version_ | 1878403939357949952 |
|---|---|
| author | Ballatore, María Belén |
| author2 | Durantini, Javier Esteban Gsponer, Natalia Soledad Suárez Ramanzin, Maria Belen Gervaldo, Miguel Andres Otero, Luis Alberto Spesia, Mariana Belen Milanesio, María Elisa Durantini, Edgardo Néstor |
| author2_role | author author author author author author author author |
| author_browse | Ballatore, María Belén Durantini, Edgardo Néstor Durantini, Javier Esteban Gervaldo, Miguel Andres Gsponer, Natalia Soledad Milanesio, María Elisa Otero, Luis Alberto Spesia, Mariana Belen Suárez Ramanzin, Maria Belen |
| author_facet | Ballatore, María Belén Durantini, Javier Esteban Gsponer, Natalia Soledad Suárez Ramanzin, Maria Belen Gervaldo, Miguel Andres Otero, Luis Alberto Spesia, Mariana Belen Milanesio, María Elisa Durantini, Edgardo Néstor |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | A porphyrin-fullerene C60 dyad (TCP-C60) substituted by carbazoyl groups was used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers, holding fullerene units. The properties of this film were compared with those formed by layers of TCP/TCP-C60 and TCP/ZnTCP. Absorption spectra of the films presented the Soret and Q bands of the corresponding porphyrins. The TCP-C60 film produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1δg) production in water. In addition, the TCP-C60 film induced the reduction of nitro blue tetrazolium to diformazan in the presence of NADH, indicating the formation of superoxide anion radical. Moreover, photooxidation of L-tryptophan mediated by TCP-C60 films was found in water. In biological media, photoinactivation of Staphylococcus aureus was evaluated depositing a drop with 2.5 × 103 cells on the films. After 30 min irradiation, no colony formation was detected using TCP-C60 or TCP/TCP-C60 films. Furthermore, photocytotoxic activity was observed in cell suspensions of S. aureus and Escherichia coli. The irradiated TCP-C60 film produced a 4 log decrease of S. aureus survival after 30 min. Also, a 4 log reduction of E. coli viability was obtained using the TCP-C60 film after 60 min irradiation. Therefore, the TCP-C60 film is an interesting and versatile photodynamic active surface to eradicate bacteria. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_dcb87beef1be4e8a229792e8ebfbf5da |
| identifier_str_mv | Ballatore, María Belén; Durantini, Javier Esteban; Gsponer, Natalia Soledad; Suárez Ramanzin, Maria Belen; Gervaldo, Miguel Andres; et al.; Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films; American Chemical Society; Environmental Science & Technology; 49; 12; 5-2015; 7456-7463 0013-936X CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/180210 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films Ballatore, María Belén Durantini, Javier Esteban Gsponer, Natalia Soledad Suárez Ramanzin, Maria Belen Gervaldo, Miguel Andres Otero, Luis Alberto Spesia, Mariana Belen Milanesio, María Elisa Durantini, Edgardo Néstor POLYMERIC FILMS PORPHYRIN FULLERENE DYAD PHOTODYNAMIC INACTIVATION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 A porphyrin-fullerene C60 dyad (TCP-C60) substituted by carbazoyl groups was used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers, holding fullerene units. The properties of this film were compared with those formed by layers of TCP/TCP-C60 and TCP/ZnTCP. Absorption spectra of the films presented the Soret and Q bands of the corresponding porphyrins. The TCP-C60 film produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1δg) production in water. In addition, the TCP-C60 film induced the reduction of nitro blue tetrazolium to diformazan in the presence of NADH, indicating the formation of superoxide anion radical. Moreover, photooxidation of L-tryptophan mediated by TCP-C60 films was found in water. In biological media, photoinactivation of Staphylococcus aureus was evaluated depositing a drop with 2.5 × 103 cells on the films. After 30 min irradiation, no colony formation was detected using TCP-C60 or TCP/TCP-C60 films. Furthermore, photocytotoxic activity was observed in cell suspensions of S. aureus and Escherichia coli. The irradiated TCP-C60 film produced a 4 log decrease of S. aureus survival after 30 min. Also, a 4 log reduction of E. coli viability was obtained using the TCP-C60 film after 60 min irradiation. Therefore, the TCP-C60 film is an interesting and versatile photodynamic active surface to eradicate bacteria. Fil: Ballatore, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Durantini, Javier Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Gsponer, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Suárez Ramanzin, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Gervaldo, Miguel Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Otero, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Spesia, Mariana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Milanesio, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina American Chemical Society http://hdl.handle.net/11336/180210 Ballatore, María Belén; Durantini, Javier Esteban; Gsponer, Natalia Soledad; Suárez Ramanzin, Maria Belen; Gervaldo, Miguel Andres; et al.; Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films; American Chemical Society; Environmental Science & Technology; 49; 12; 5-2015; 7456-7463 0013-936X CONICET Digital CONICET |
| spellingShingle | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films Ballatore, María Belén POLYMERIC FILMS PORPHYRIN FULLERENE DYAD PHOTODYNAMIC INACTIVATION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films |
| title_full | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films |
| title_fullStr | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films |
| title_full_unstemmed | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films |
| title_short | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films |
| title_sort | Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films |
| topic | POLYMERIC FILMS PORPHYRIN FULLERENE DYAD PHOTODYNAMIC INACTIVATION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| topic_facet | POLYMERIC FILMS PORPHYRIN FULLERENE DYAD PHOTODYNAMIC INACTIVATION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/180210 |