Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
Arginine kinase catalyzes the transphosphorylation between phosphoarginine and ADP. Phosphoarginine is involved in temporal ATP buffering and inorganic phosphate regulation. Trypanosoma cruzi arginine kinase phosphorylates only L‐arginine (specific activity 398.9 mUE‐min−1 mg−1), and is inhibited by...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2003 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/43567 |
| Online Access: | http://hdl.handle.net/11336/43567 |
| Access Level: | Open access |
| Keyword: | Canavanine Guanidino Kinase Homoarginine Phosphagen Kinase Phosphoarginine https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403927658987520 |
|---|---|
| author | Pereira, Claudio Alejandro |
| author2 | Alonso, Guillermo Daniel Ivaldi, María Soledad Bouvier, León Alberto Torres, Hector Norberto Flawia, Mirtha Maria |
| author2_role | author author author author author |
| author_browse | Alonso, Guillermo Daniel Bouvier, León Alberto Flawia, Mirtha Maria Ivaldi, María Soledad Pereira, Claudio Alejandro Torres, Hector Norberto |
| author_facet | Pereira, Claudio Alejandro Alonso, Guillermo Daniel Ivaldi, María Soledad Bouvier, León Alberto Torres, Hector Norberto Flawia, Mirtha Maria |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Arginine kinase catalyzes the transphosphorylation between phosphoarginine and ADP. Phosphoarginine is involved in temporal ATP buffering and inorganic phosphate regulation. Trypanosoma cruzi arginine kinase phosphorylates only L‐arginine (specific activity 398.9 mUE‐min−1 mg−1), and is inhibited by the arginine analogs, agmatine, canavanine, nitroarginine, and homoarginine. Canavanine and homoarginine also produce a significant inhibition of the epimastigote culture growth (79.7% and 55.8%, respectively). Inhibition constants were calculated for canavanine and homoarginine (7.55 and 6.02 mM, respectively). In addition, two novel guanidino kinase activities were detected in the epimastigote soluble extract. The development of the arginine kinase inhibitors of T. cruzi could be an important feature because the phosphagens biosynthetic pathway in trypanosomatids is different from the one in their mammalian hosts. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_daa697e54e1eb73827ab7466e5c6d40d |
| identifier_str_mv | Pereira, Claudio Alejandro; Alonso, Guillermo Daniel; Ivaldi, María Soledad; Bouvier, León Alberto; Torres, Hector Norberto; et al.; Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 50; 2; 12-2003; 132-134 1066-5234 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/43567 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | Wiley Blackwell Publishing, Inc |
| publisherStr | Wiley Blackwell Publishing, Inc |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi Pereira, Claudio Alejandro Alonso, Guillermo Daniel Ivaldi, María Soledad Bouvier, León Alberto Torres, Hector Norberto Flawia, Mirtha Maria Canavanine Guanidino Kinase Homoarginine Phosphagen Kinase Phosphoarginine https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Arginine kinase catalyzes the transphosphorylation between phosphoarginine and ADP. Phosphoarginine is involved in temporal ATP buffering and inorganic phosphate regulation. Trypanosoma cruzi arginine kinase phosphorylates only L‐arginine (specific activity 398.9 mUE‐min−1 mg−1), and is inhibited by the arginine analogs, agmatine, canavanine, nitroarginine, and homoarginine. Canavanine and homoarginine also produce a significant inhibition of the epimastigote culture growth (79.7% and 55.8%, respectively). Inhibition constants were calculated for canavanine and homoarginine (7.55 and 6.02 mM, respectively). In addition, two novel guanidino kinase activities were detected in the epimastigote soluble extract. The development of the arginine kinase inhibitors of T. cruzi could be an important feature because the phosphagens biosynthetic pathway in trypanosomatids is different from the one in their mammalian hosts. Fil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Ivaldi, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Torres, Hector Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Flawia, Mirtha Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Wiley Blackwell Publishing, Inc http://hdl.handle.net/11336/43567 Pereira, Claudio Alejandro; Alonso, Guillermo Daniel; Ivaldi, María Soledad; Bouvier, León Alberto; Torres, Hector Norberto; et al.; Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 50; 2; 12-2003; 132-134 1066-5234 CONICET Digital CONICET |
| spellingShingle | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi Pereira, Claudio Alejandro Canavanine Guanidino Kinase Homoarginine Phosphagen Kinase Phosphoarginine https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi |
| title_full | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi |
| title_fullStr | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi |
| title_full_unstemmed | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi |
| title_short | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi |
| title_sort | Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi |
| topic | Canavanine Guanidino Kinase Homoarginine Phosphagen Kinase Phosphoarginine https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | Canavanine Guanidino Kinase Homoarginine Phosphagen Kinase Phosphoarginine https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/43567 |