Precision phenotyping of imidazolinone-induced chlorosis in sunflower
Chlorosis level is a useful parameter to assess imidazolinone resistance in sunflower (Helianthus annuus L.). The aim of this study was to quantify chlorosis through two different methods in sunflower plantlets treated with imazapyr. The genotypes used in this study were two inbred lines reported to...
| Authors: | ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2014 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/180886 |
| Online Access: | http://hdl.handle.net/11336/180886 |
| Access Level: | Open access |
| Keyword: | Carotenoids Chlorophylls Chlorosis Color analysis software Helianthus annuus L. Sunflower https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403882223140864 |
|---|---|
| author | Ochogavía, Ana Claudia |
| author2 | Gil, Mercedes Picardi, Liliana Amelia Nestares, Graciela María |
| author2_role | author author author |
| author_browse | Gil, Mercedes Nestares, Graciela María Ochogavía, Ana Claudia Picardi, Liliana Amelia |
| author_facet | Ochogavía, Ana Claudia Gil, Mercedes Picardi, Liliana Amelia Nestares, Graciela María |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Chlorosis level is a useful parameter to assess imidazolinone resistance in sunflower (Helianthus annuus L.). The aim of this study was to quantify chlorosis through two different methods in sunflower plantlets treated with imazapyr. The genotypes used in this study were two inbred lines reported to be different in their resistance to imidazolinones. Chlorosis was evaluated by spectrophotometrical quantification of photosynthetic leaf pigments and by a bioinformatics-based color analysis. A protocol for pigment extraction was presented which improved pigment stability. Chlorophyll amount decreased significantly when both genotypes were treated with 10 μM of imazapyr. Leaf color was characterized using Tomato Analyzer® color test software. A significant positive correlation between color reduction and chlorophyll concentration was found. It suggests that leaf color measurement could be an accurate method to estimate chlorosis and infer chlorophyll levels in sunflower plants. These results highlight a strong relationship between imidazolinone-induced chlorosis and variations in leaf color and in chlorophyll concentration. Both methods are quantitative, rapid, simple, and reproducible. Thus, they could be useful tools for phenotyping and screening large number of plants when breeding for imidazolinone resistance in this species. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_d1bb4ff8d0ff8a5d7f3c0db97c3ec84e |
| identifier_str_mv | Ochogavía, Ana Claudia; Gil, Mercedes; Picardi, Liliana Amelia; Nestares, Graciela María; Precision phenotyping of imidazolinone-induced chlorosis in sunflower; Japanese Society of Breeding; Breeding Science; 64; 4; 1-2014; 416-421 1344-7610 1347-3735 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/180886 |
| publishDate | 2014 |
| publishDateSort | 2014 |
| publisher | Japanese Society of Breeding |
| publisherStr | Japanese Society of Breeding |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Precision phenotyping of imidazolinone-induced chlorosis in sunflower Ochogavía, Ana Claudia Gil, Mercedes Picardi, Liliana Amelia Nestares, Graciela María Carotenoids Chlorophylls Chlorosis Color analysis software Helianthus annuus L. Sunflower https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Chlorosis level is a useful parameter to assess imidazolinone resistance in sunflower (Helianthus annuus L.). The aim of this study was to quantify chlorosis through two different methods in sunflower plantlets treated with imazapyr. The genotypes used in this study were two inbred lines reported to be different in their resistance to imidazolinones. Chlorosis was evaluated by spectrophotometrical quantification of photosynthetic leaf pigments and by a bioinformatics-based color analysis. A protocol for pigment extraction was presented which improved pigment stability. Chlorophyll amount decreased significantly when both genotypes were treated with 10 μM of imazapyr. Leaf color was characterized using Tomato Analyzer® color test software. A significant positive correlation between color reduction and chlorophyll concentration was found. It suggests that leaf color measurement could be an accurate method to estimate chlorosis and infer chlorophyll levels in sunflower plants. These results highlight a strong relationship between imidazolinone-induced chlorosis and variations in leaf color and in chlorophyll concentration. Both methods are quantitative, rapid, simple, and reproducible. Thus, they could be useful tools for phenotyping and screening large number of plants when breeding for imidazolinone resistance in this species. Fil: Ochogavía, Ana Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Gil, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Picardi, Liliana Amelia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina Fil: Nestares, Graciela María. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Japanese Society of Breeding http://hdl.handle.net/11336/180886 Ochogavía, Ana Claudia; Gil, Mercedes; Picardi, Liliana Amelia; Nestares, Graciela María; Precision phenotyping of imidazolinone-induced chlorosis in sunflower; Japanese Society of Breeding; Breeding Science; 64; 4; 1-2014; 416-421 1344-7610 1347-3735 CONICET Digital CONICET |
| spellingShingle | Precision phenotyping of imidazolinone-induced chlorosis in sunflower Ochogavía, Ana Claudia Carotenoids Chlorophylls Chlorosis Color analysis software Helianthus annuus L. Sunflower https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Precision phenotyping of imidazolinone-induced chlorosis in sunflower |
| title_full | Precision phenotyping of imidazolinone-induced chlorosis in sunflower |
| title_fullStr | Precision phenotyping of imidazolinone-induced chlorosis in sunflower |
| title_full_unstemmed | Precision phenotyping of imidazolinone-induced chlorosis in sunflower |
| title_short | Precision phenotyping of imidazolinone-induced chlorosis in sunflower |
| title_sort | Precision phenotyping of imidazolinone-induced chlorosis in sunflower |
| topic | Carotenoids Chlorophylls Chlorosis Color analysis software Helianthus annuus L. Sunflower https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | Carotenoids Chlorophylls Chlorosis Color analysis software Helianthus annuus L. Sunflower https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/180886 |