A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2018 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/96846 |
| Online Access: | http://hdl.handle.net/11336/96846 |
| Access Level: | Open access |
| Keyword: | Dendritic Spines MAP6 Actin Neurons https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403869984161792 |
|---|---|
| author | Peris, Leticia |
| author2 | Bisbal, Mariano Martinez Hernandez, José Saoudi, Yasmina Jonckheere, Julie Rolland, Marta Sebastien, Muriel Brocard, Jacques Denarier, Eric Bosc, Christopher Guerin, Christophe Gory Fauré, Sylvie Deloulme, Jean Christophe Lanté, Fabien Arnal, Isabelle Buisson, Alain Goldberg, Yves Blanchoin, Laurent Delphin, Christian Andrieux, Annie |
| author2_role | author author author author author author author author author author author author author author author author author author author |
| author_browse | Andrieux, Annie Arnal, Isabelle Bisbal, Mariano Blanchoin, Laurent Bosc, Christopher Brocard, Jacques Buisson, Alain Deloulme, Jean Christophe Delphin, Christian Denarier, Eric Goldberg, Yves Gory Fauré, Sylvie Guerin, Christophe Jonckheere, Julie Lanté, Fabien Martinez Hernandez, José Peris, Leticia Rolland, Marta Saoudi, Yasmina Sebastien, Muriel |
| author_facet | Peris, Leticia Bisbal, Mariano Martinez Hernandez, José Saoudi, Yasmina Jonckheere, Julie Rolland, Marta Sebastien, Muriel Brocard, Jacques Denarier, Eric Bosc, Christopher Guerin, Christophe Gory Fauré, Sylvie Deloulme, Jean Christophe Lanté, Fabien Arnal, Isabelle Buisson, Alain Goldberg, Yves Blanchoin, Laurent Delphin, Christian Andrieux, Annie |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_d00a58ff2c6c8e44c6d6149441d6006a |
| identifier_str_mv | Peris, Leticia; Bisbal, Mariano; Martinez Hernandez, José; Saoudi, Yasmina; Jonckheere, Julie; et al.; A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-15 2041-1723 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/96846 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | Nature Publishing Group |
| publisherStr | Nature Publishing Group |
| reponame_str | CONICET Digital (CONICET) |
| spelling | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines Peris, Leticia Bisbal, Mariano Martinez Hernandez, José Saoudi, Yasmina Jonckheere, Julie Rolland, Marta Sebastien, Muriel Brocard, Jacques Denarier, Eric Bosc, Christopher Guerin, Christophe Gory Fauré, Sylvie Deloulme, Jean Christophe Lanté, Fabien Arnal, Isabelle Buisson, Alain Goldberg, Yves Blanchoin, Laurent Delphin, Christian Andrieux, Annie Dendritic Spines MAP6 Actin Neurons https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses. Fil: Peris, Leticia. Universite Grenoble Alpes; Francia Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universite Grenoble Alpes; Francia Fil: Martinez Hernandez, José. Universidad del País Vasco; España. Universite Grenoble Alpes; Francia Fil: Saoudi, Yasmina. Universite Grenoble Alpes; Francia Fil: Jonckheere, Julie. Universite Grenoble Alpes; Francia Fil: Rolland, Marta. Universite Grenoble Alpes; Francia Fil: Sebastien, Muriel. Universite Grenoble Alpes; Francia Fil: Brocard, Jacques. Universite Grenoble Alpes; Francia Fil: Denarier, Eric. Universite Grenoble Alpes; Francia Fil: Bosc, Christopher. Universite Grenoble Alpes; Francia Fil: Guerin, Christophe. Universite Grenoble Alpes; Francia. Inserm; Francia Fil: Gory Fauré, Sylvie. Universite Grenoble Alpes; Francia Fil: Deloulme, Jean Christophe. Universite Grenoble Alpes; Francia Fil: Lanté, Fabien. Universite Grenoble Alpes; Francia Fil: Arnal, Isabelle. Universite Grenoble Alpes; Francia Fil: Buisson, Alain. Universite Grenoble Alpes; Francia Fil: Goldberg, Yves. Universite Grenoble Alpes; Francia Fil: Blanchoin, Laurent. Inserm; Francia. Universite Grenoble Alpes; Francia Fil: Delphin, Christian. Universite Grenoble Alpes; Francia Fil: Andrieux, Annie. Universite Grenoble Alpes; Francia Nature Publishing Group http://hdl.handle.net/11336/96846 Peris, Leticia; Bisbal, Mariano; Martinez Hernandez, José; Saoudi, Yasmina; Jonckheere, Julie; et al.; A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-15 2041-1723 CONICET Digital CONICET |
| spellingShingle | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines Peris, Leticia Dendritic Spines MAP6 Actin Neurons https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines |
| title_full | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines |
| title_fullStr | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines |
| title_full_unstemmed | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines |
| title_short | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines |
| title_sort | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines |
| topic | Dendritic Spines MAP6 Actin Neurons https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | Dendritic Spines MAP6 Actin Neurons https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/96846 |