Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In t...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2023 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/226369 |
| Online Access: | http://hdl.handle.net/11336/226369 |
| Access Level: | Open access |
| Keyword: | METATRANSCRIPTOMICS OPHIOVIRUS PLANT VIRUSES VIRUS DISCOVERY VIRUS TAXONOMY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403864791613440 |
|---|---|
| author | Debat, Humberto Julio |
| author2 | Garcia, Maria Laura Bejerman, Nicolas |
| author2_role | author author |
| author_browse | Bejerman, Nicolas Debat, Humberto Julio Garcia, Maria Laura |
| author_facet | Debat, Humberto Julio Garcia, Maria Laura Bejerman, Nicolas |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_cebb4f00a9f4c0426ea3652b3af31df9 |
| identifier_str_mv | Debat, Humberto Julio; Garcia, Maria Laura; Bejerman, Nicolas; Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data; MDPI; Viruses; 15; 4; 3-2023; 1-21 1999-4915 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/226369 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data Debat, Humberto Julio Garcia, Maria Laura Bejerman, Nicolas METATRANSCRIPTOMICS OPHIOVIRUS PLANT VIRUSES VIRUS DISCOVERY VIRUS TAXONOMY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentina Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentina MDPI http://hdl.handle.net/11336/226369 Debat, Humberto Julio; Garcia, Maria Laura; Bejerman, Nicolas; Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data; MDPI; Viruses; 15; 4; 3-2023; 1-21 1999-4915 CONICET Digital CONICET |
| spellingShingle | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data Debat, Humberto Julio METATRANSCRIPTOMICS OPHIOVIRUS PLANT VIRUSES VIRUS DISCOVERY VIRUS TAXONOMY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_full | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_fullStr | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_full_unstemmed | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_short | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_sort | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| topic | METATRANSCRIPTOMICS OPHIOVIRUS PLANT VIRUSES VIRUS DISCOVERY VIRUS TAXONOMY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | METATRANSCRIPTOMICS OPHIOVIRUS PLANT VIRUSES VIRUS DISCOVERY VIRUS TAXONOMY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/226369 |