Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from to...
| Authors: | ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2020 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/146046 |
| Online Access: | http://hdl.handle.net/11336/146046 |
| Access Level: | Open access |
| Keyword: | ENDOPHYTES N2-FIXING BACTERIA NIFH GENE PYROSEQUENCING TOMATO https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
| _version_ | 1878403645920247808 |
|---|---|
| author | Collavino, Mónica Mariana |
| author2 | Ramos Cabrera, Efren Venancio Bruno, Cecilia Ines Aguilar, Orlando Mario |
| author2_role | author author author |
| author_browse | Aguilar, Orlando Mario Bruno, Cecilia Ines Collavino, Mónica Mariana Ramos Cabrera, Efren Venancio |
| author_facet | Collavino, Mónica Mariana Ramos Cabrera, Efren Venancio Bruno, Cecilia Ines Aguilar, Orlando Mario |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_a5cb4e2fe5f467f25bfb8ecbdd37cf32 |
| identifier_str_mv | Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-1975 1517-8382 1678-4405 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/146046 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth Collavino, Mónica Mariana Ramos Cabrera, Efren Venancio Bruno, Cecilia Ines Aguilar, Orlando Mario ENDOPHYTES N2-FIXING BACTERIA NIFH GENE PYROSEQUENCING TOMATO https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops. Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Ramos Cabrera, Efren Venancio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Bruno, Cecilia Ines. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; Argentina Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Springer http://hdl.handle.net/11336/146046 Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-1975 1517-8382 1678-4405 CONICET Digital CONICET |
| spellingShingle | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth Collavino, Mónica Mariana ENDOPHYTES N2-FIXING BACTERIA NIFH GENE PYROSEQUENCING TOMATO https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
| status_str | publishedVersion |
| title | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
| title_full | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
| title_fullStr | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
| title_full_unstemmed | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
| title_short | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
| title_sort | Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
| topic | ENDOPHYTES N2-FIXING BACTERIA NIFH GENE PYROSEQUENCING TOMATO https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
| topic_facet | ENDOPHYTES N2-FIXING BACTERIA NIFH GENE PYROSEQUENCING TOMATO https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
| url | http://hdl.handle.net/11336/146046 |