Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)

The objective of this work was to evaluate the in vivo acetohydroxyacid synthase (AHAS) activity response to imidazolinones and its possible use as a selection method for evaluating AHAS inhibitor resistance. In vivo AHAS assay and the comparison of parameters from dose-response curves have been use...

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Authors: Vega, Tatiana Alejandra; Breccia, Gabriela; Gil, Mercedes; Zorzoli, Roxana; Picardi, L.; Nestares, Graciela María
Format: Article
Status:Published version
Publication date:2012
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/139160
Online Access:http://hdl.handle.net/11336/139160
Access Level:Open access
Keyword:AHAS INHIBITORS
HELIANTHUS ANNUUS L
IMIDAZOLINONE
IN VIVO AHAS ACTIVITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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author Vega, Tatiana Alejandra
author2 Breccia, Gabriela
Gil, Mercedes
Zorzoli, Roxana
Picardi, L.
Nestares, Graciela María
author2_role author
author
author
author
author
author_browse Breccia, Gabriela
Gil, Mercedes
Nestares, Graciela María
Picardi, L.
Vega, Tatiana Alejandra
Zorzoli, Roxana
author_facet Vega, Tatiana Alejandra
Breccia, Gabriela
Gil, Mercedes
Zorzoli, Roxana
Picardi, L.
Nestares, Graciela María
author_role author
collection CONICET Digital (CONICET)
description The objective of this work was to evaluate the in vivo acetohydroxyacid synthase (AHAS) activity response to imidazolinones and its possible use as a selection method for evaluating AHAS inhibitor resistance. In vivo AHAS assay and the comparison of parameters from dose-response curves have been used as a valid tool for comparing sunflower lines and hybrids differing in imidazolinone resistance. The sunflower resistant genotypes evaluated here were 100-fold and 20-fold more resistant compared with the susceptible line for imazethapyr and imazapyr, respectively. This assay also allowed discrimination of homozygous from heterozygous genotypes for Imr1 locus that codify for the catalytic subunit of AHAS. The in vivo AHAS assay described in this study was useful for the selection of sunflower genotypes differing in herbicide resistance and could be a useful tool when breeding for imidazolinone resistance in sunflower.
eu_rights_str_mv openAccess
format article
id AR_9812f4b1e4e00a4d069a4a183b730f61
identifier_str_mv Vega, Tatiana Alejandra; Breccia, Gabriela; Gil, Mercedes; Zorzoli, Roxana; Picardi, L.; et al.; Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.); Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 61; 12-2012; 103-107
0981-9428
CONICET Digital
CONICET
institution Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
language eng
network_acronym_str AR
network_name_str Argentina
oai_identifier_str oai:ri.conicet.gov.ar:11336/139160
publishDate 2012
publishDateSort 2012
publisher Elsevier France-Editions Scientifiques Medicales Elsevier
publisherStr Elsevier France-Editions Scientifiques Medicales Elsevier
reponame_str CONICET Digital (CONICET)
spelling Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.) Vega, Tatiana Alejandra Breccia, Gabriela Gil, Mercedes Zorzoli, Roxana Picardi, L. Nestares, Graciela María AHAS INHIBITORS HELIANTHUS ANNUUS L IMIDAZOLINONE IN VIVO AHAS ACTIVITY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 The objective of this work was to evaluate the in vivo acetohydroxyacid synthase (AHAS) activity response to imidazolinones and its possible use as a selection method for evaluating AHAS inhibitor resistance. In vivo AHAS assay and the comparison of parameters from dose-response curves have been used as a valid tool for comparing sunflower lines and hybrids differing in imidazolinone resistance. The sunflower resistant genotypes evaluated here were 100-fold and 20-fold more resistant compared with the susceptible line for imazethapyr and imazapyr, respectively. This assay also allowed discrimination of homozygous from heterozygous genotypes for Imr1 locus that codify for the catalytic subunit of AHAS. The in vivo AHAS assay described in this study was useful for the selection of sunflower genotypes differing in herbicide resistance and could be a useful tool when breeding for imidazolinone resistance in sunflower. Fil: Vega, Tatiana Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Breccia, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina Fil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina Fil: Zorzoli, Roxana. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina Fil: Picardi, L.. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina Fil: Nestares, Graciela María. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Elsevier France-Editions Scientifiques Medicales Elsevier http://hdl.handle.net/11336/139160 Vega, Tatiana Alejandra; Breccia, Gabriela; Gil, Mercedes; Zorzoli, Roxana; Picardi, L.; et al.; Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.); Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 61; 12-2012; 103-107 0981-9428 CONICET Digital CONICET
spellingShingle Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
Vega, Tatiana Alejandra
AHAS INHIBITORS
HELIANTHUS ANNUUS L
IMIDAZOLINONE
IN VIVO AHAS ACTIVITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
status_str publishedVersion
title Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
title_full Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
title_fullStr Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
title_full_unstemmed Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
title_short Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
title_sort Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
topic AHAS INHIBITORS
HELIANTHUS ANNUUS L
IMIDAZOLINONE
IN VIVO AHAS ACTIVITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic_facet AHAS INHIBITORS
HELIANTHUS ANNUUS L
IMIDAZOLINONE
IN VIVO AHAS ACTIVITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
url http://hdl.handle.net/11336/139160

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