Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens
Bovine mastitis, considered the most important cause of economic losses in the dairy industry, is a major concern in veterinary medicine. Staphylococcus aureus and coagulase-negative staphylococci (CNS) are the main pathogens associated with intramammary infections, and bacterial biofilms are suspec...
| Autores: | ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artigo |
| Estado: | Published version |
| Data de publicação: | 2017 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | Inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/137605 |
| Acesso em linha: | http://hdl.handle.net/11336/137605 |
| Nível de acesso: | Acesso aberto |
| Palavra-chave: | ANTI-BIOFILM COMPOUNDS COMMENSAL BACTERIA MASTITIS UDDER HEALTH https://purl.org/becyt/ford/4.2 https://purl.org/becyt/ford/4 |
| _version_ | 1878403544835424256 |
|---|---|
| author | Isaac, Paula |
| author2 | Bohl, Luciana Paola Breser, Maria Laura Orellano, María Soledad Conesa, Agustin Ferrero, Marcela Alejandra Porporatto, Carina |
| author2_role | author author author author author author |
| author_browse | Bohl, Luciana Paola Breser, Maria Laura Conesa, Agustin Ferrero, Marcela Alejandra Isaac, Paula Orellano, María Soledad Porporatto, Carina |
| author_facet | Isaac, Paula Bohl, Luciana Paola Breser, Maria Laura Orellano, María Soledad Conesa, Agustin Ferrero, Marcela Alejandra Porporatto, Carina |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Bovine mastitis, considered the most important cause of economic losses in the dairy industry, is a major concern in veterinary medicine. Staphylococcus aureus and coagulase-negative staphylococci (CNS) are the main pathogens associated with intramammary infections, and bacterial biofilms are suspected to be responsible for the persistence of this disease. CNS from the udder are not necessarily associated with intramammary infections. In fact, some commensal CNS have been shown to have biological activities. This issue led us to screen exoproducts from commensal Staphylococcus chromogenes for anti-biofilm activity against different mastitis pathogens. The cell-free supernatant from S. chromogenes LN1 (LN1-CFS) was confirmed to display a non-biocidal inhibition of pathogenic biofilms. The supernatant was subjected to various treatments to estimate the nature of the biofilm-inhibiting compounds. The results showed that the bioactive compound >5 KDa in mass is sensitive to thermal treatment and proteinase K digestion, suggesting its protein properties. LN1-CFS was able to significantly inhibit S. aureus and CNS biofilm formation in a dose-independent manner and without affecting the viability of bovine cells. These findings reveal a new activity of the udder microflora of healthy animals. Studies are underway to purify and identify the anti-biofilm biocompound and to evaluate its biological activity in vivo. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_928cd7f9ecc49aab5b1e85c60b3e2a65 |
| identifier_str_mv | Isaac, Paula; Bohl, Luciana Paola; Breser, Maria Laura; Orellano, María Soledad; Conesa, Agustin; et al.; Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens; Elsevier Science; Veterinary Microbiology; 207; 8-2017; 259-266 0378-1135 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/137605 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | Elsevier Science |
| publisherStr | Elsevier Science |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens Isaac, Paula Bohl, Luciana Paola Breser, Maria Laura Orellano, María Soledad Conesa, Agustin Ferrero, Marcela Alejandra Porporatto, Carina ANTI-BIOFILM COMPOUNDS COMMENSAL BACTERIA MASTITIS UDDER HEALTH https://purl.org/becyt/ford/4.2 https://purl.org/becyt/ford/4 Bovine mastitis, considered the most important cause of economic losses in the dairy industry, is a major concern in veterinary medicine. Staphylococcus aureus and coagulase-negative staphylococci (CNS) are the main pathogens associated with intramammary infections, and bacterial biofilms are suspected to be responsible for the persistence of this disease. CNS from the udder are not necessarily associated with intramammary infections. In fact, some commensal CNS have been shown to have biological activities. This issue led us to screen exoproducts from commensal Staphylococcus chromogenes for anti-biofilm activity against different mastitis pathogens. The cell-free supernatant from S. chromogenes LN1 (LN1-CFS) was confirmed to display a non-biocidal inhibition of pathogenic biofilms. The supernatant was subjected to various treatments to estimate the nature of the biofilm-inhibiting compounds. The results showed that the bioactive compound >5 KDa in mass is sensitive to thermal treatment and proteinase K digestion, suggesting its protein properties. LN1-CFS was able to significantly inhibit S. aureus and CNS biofilm formation in a dose-independent manner and without affecting the viability of bovine cells. These findings reveal a new activity of the udder microflora of healthy animals. Studies are underway to purify and identify the anti-biofilm biocompound and to evaluate its biological activity in vivo. Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Fil: Bohl, Luciana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Fil: Breser, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Fil: Orellano, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Fil: Conesa, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Fil: Ferrero, Marcela Alejandra. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Porporatto, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Elsevier Science http://hdl.handle.net/11336/137605 Isaac, Paula; Bohl, Luciana Paola; Breser, Maria Laura; Orellano, María Soledad; Conesa, Agustin; et al.; Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens; Elsevier Science; Veterinary Microbiology; 207; 8-2017; 259-266 0378-1135 CONICET Digital CONICET |
| spellingShingle | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens Isaac, Paula ANTI-BIOFILM COMPOUNDS COMMENSAL BACTERIA MASTITIS UDDER HEALTH https://purl.org/becyt/ford/4.2 https://purl.org/becyt/ford/4 |
| status_str | publishedVersion |
| title | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens |
| title_full | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens |
| title_fullStr | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens |
| title_full_unstemmed | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens |
| title_short | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens |
| title_sort | Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens |
| topic | ANTI-BIOFILM COMPOUNDS COMMENSAL BACTERIA MASTITIS UDDER HEALTH https://purl.org/becyt/ford/4.2 https://purl.org/becyt/ford/4 |
| topic_facet | ANTI-BIOFILM COMPOUNDS COMMENSAL BACTERIA MASTITIS UDDER HEALTH https://purl.org/becyt/ford/4.2 https://purl.org/becyt/ford/4 |
| url | http://hdl.handle.net/11336/137605 |