Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
VHDL fraction contains hemocyanin as its major apoprotein and transports most of the circulating lipids in the spider Polybetes pythagoricus (Sparassidae). This work shows that subfraction II (the major VHDL component) is composed of a single protein of 420 kDa under native conditions and three subu...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2015 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/9064 |
| Online Access: | http://hdl.handle.net/11336/9064 |
| Access Level: | Open access |
| Keyword: | HEMOCYANIN MALDI-TOF LIPIDS FLUORESCENCE ARACHNID HEXAMER https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403185581752320 |
|---|---|
| author | Laino, Aldana |
| author2 | Garcia, Fernando Cunningham, Monica Liliana |
| author2_role | author author |
| author_browse | Cunningham, Monica Liliana Garcia, Fernando Laino, Aldana |
| author_facet | Laino, Aldana Garcia, Fernando Cunningham, Monica Liliana |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | VHDL fraction contains hemocyanin as its major apoprotein and transports most of the circulating lipids in the spider Polybetes pythagoricus (Sparassidae). This work shows that subfraction II (the major VHDL component) is composed of a single protein of 420 kDa under native conditions and three subunits, 67, 105 and 121 kDa under denaturing conditions. Circular dichroism indicated that this subfraction contains 20% α-helix, 29% β-sheet, 22.7% turns and 29.7% unordered structures. Comparison of trypsin susceptibility showed that the 105 and 121 kDa subunits were more susceptible indicating that these proteins would be more exposed to the aqueous medium. Peptide mass fingerprinting of the 67 and 105 kDa subunits indicated the 67 kDa subunit is similar to subunit 3 of the spider Cupiennius salei hemocyanin (21% sequence similarity), whereas the 105 kDa subunit is similar to a protein from the mosquito Anopheles gambiae (20% sequence similarity). The N-terminal amino acid sequence from subunit of 121 kDa was also determined. In relation to fatty acids, 16:0, 18:0, 18:1 and 18:2 were found to be the major components. These data provide a better understanding of VHDL subfraction II structure, which is responsible for most lipid transport in the spider P. pythagoricus. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_4e29d9aecb6a38cfd93bb29e7b9efeaf |
| identifier_str_mv | Laino, Aldana; Garcia, Fernando; Cunningham, Monica Liliana; Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus; Inst Histol Embriol-conicet; Biocell; 39; 1; 6-2015; 33-40 0327-9545 1667-5746 |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/9064 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher | Inst Histol Embriol-conicet |
| publisherStr | Inst Histol Embriol-conicet |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus Laino, Aldana Garcia, Fernando Cunningham, Monica Liliana HEMOCYANIN MALDI-TOF LIPIDS FLUORESCENCE ARACHNID HEXAMER https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 VHDL fraction contains hemocyanin as its major apoprotein and transports most of the circulating lipids in the spider Polybetes pythagoricus (Sparassidae). This work shows that subfraction II (the major VHDL component) is composed of a single protein of 420 kDa under native conditions and three subunits, 67, 105 and 121 kDa under denaturing conditions. Circular dichroism indicated that this subfraction contains 20% α-helix, 29% β-sheet, 22.7% turns and 29.7% unordered structures. Comparison of trypsin susceptibility showed that the 105 and 121 kDa subunits were more susceptible indicating that these proteins would be more exposed to the aqueous medium. Peptide mass fingerprinting of the 67 and 105 kDa subunits indicated the 67 kDa subunit is similar to subunit 3 of the spider Cupiennius salei hemocyanin (21% sequence similarity), whereas the 105 kDa subunit is similar to a protein from the mosquito Anopheles gambiae (20% sequence similarity). The N-terminal amino acid sequence from subunit of 121 kDa was also determined. In relation to fatty acids, 16:0, 18:0, 18:1 and 18:2 were found to be the major components. These data provide a better understanding of VHDL subfraction II structure, which is responsible for most lipid transport in the spider P. pythagoricus. Fil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina Fil: Garcia, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina Fil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina Inst Histol Embriol-conicet http://hdl.handle.net/11336/9064 Laino, Aldana; Garcia, Fernando; Cunningham, Monica Liliana; Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus; Inst Histol Embriol-conicet; Biocell; 39; 1; 6-2015; 33-40 0327-9545 1667-5746 |
| spellingShingle | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus Laino, Aldana HEMOCYANIN MALDI-TOF LIPIDS FLUORESCENCE ARACHNID HEXAMER https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus |
| title_full | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus |
| title_fullStr | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus |
| title_full_unstemmed | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus |
| title_short | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus |
| title_sort | Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus |
| topic | HEMOCYANIN MALDI-TOF LIPIDS FLUORESCENCE ARACHNID HEXAMER https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | HEMOCYANIN MALDI-TOF LIPIDS FLUORESCENCE ARACHNID HEXAMER https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/9064 |