Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis
Plant ribosomal proteins play universal roles in translation, although they are also involved in developmental processes and hormone signaling pathways. Among Arabidopsis RPL10 family members, RPL10A exhibits the highest expression during germination and early development, suggesting that RPL10A is...
| Authors: | ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2020 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/147342 |
| Online Access: | http://hdl.handle.net/11336/147342 |
| Access Level: | Open access |
| Keyword: | ABA, EARLY SEEDLING DEVELOPMENT RPL10A MUTANT RPL10A-OVEREXPRESSING LINES SEED GERMINATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403185534566400 |
|---|---|
| author | Ramos, Rocío Soledad |
| author2 | Casati, Paula Spampinato, Claudia Patricia Falcone Ferreyra, María Lorena |
| author2_role | author author author |
| author_browse | Casati, Paula Falcone Ferreyra, María Lorena Ramos, Rocío Soledad Spampinato, Claudia Patricia |
| author_facet | Ramos, Rocío Soledad Casati, Paula Spampinato, Claudia Patricia Falcone Ferreyra, María Lorena |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Plant ribosomal proteins play universal roles in translation, although they are also involved in developmental processes and hormone signaling pathways. Among Arabidopsis RPL10 family members, RPL10A exhibits the highest expression during germination and early development, suggesting that RPL10A is the main contributor to these processes. In this work, we first analyzed RPL10A expression pattern in Arabidopsis thaliana using transgenic RPL10Apro:GUS plants. The gene exhibits a ubiquitous expression pattern throughout the plant, but it is most strongly expressed in undifferentiated tissues. Interestingly, gene expression was also detected in stomatal cells. We then examined protein function during seedling establishment and abscisic acid (ABA) response. Heterozygous rpl10A mutant plants show decreased ABA-sensitivity during seed germination, are impaired in early seedling and root development, and exhibit reduced ABA-inhibition of stomatal aperture under light conditions. Overexpression of RPL10A does not affect the germination and seedling growth, but RPL10A-overexpressing lines are more sensitive to ABA during early plant development and exhibit higher stomatal closure under light condition both with and without ABA treatment than wild type plants. Interestingly, RPL10A expression is induced by ABA. Together, we conclude that RPL10A could act as a positive regulator for ABA-dependent responses in Arabidopsis plants. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_4e23a6eaecac6ec553384ee4e39bac41 |
| identifier_str_mv | Ramos, Rocío Soledad; Casati, Paula; Spampinato, Claudia Patricia; Falcone Ferreyra, María Lorena; Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis; Frontiers Media; Frontiers in Plant Science; 11; 11-2020; 1-14 1664-462X CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/147342 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis Ramos, Rocío Soledad Casati, Paula Spampinato, Claudia Patricia Falcone Ferreyra, María Lorena ABA, EARLY SEEDLING DEVELOPMENT RPL10A MUTANT RPL10A-OVEREXPRESSING LINES SEED GERMINATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Plant ribosomal proteins play universal roles in translation, although they are also involved in developmental processes and hormone signaling pathways. Among Arabidopsis RPL10 family members, RPL10A exhibits the highest expression during germination and early development, suggesting that RPL10A is the main contributor to these processes. In this work, we first analyzed RPL10A expression pattern in Arabidopsis thaliana using transgenic RPL10Apro:GUS plants. The gene exhibits a ubiquitous expression pattern throughout the plant, but it is most strongly expressed in undifferentiated tissues. Interestingly, gene expression was also detected in stomatal cells. We then examined protein function during seedling establishment and abscisic acid (ABA) response. Heterozygous rpl10A mutant plants show decreased ABA-sensitivity during seed germination, are impaired in early seedling and root development, and exhibit reduced ABA-inhibition of stomatal aperture under light conditions. Overexpression of RPL10A does not affect the germination and seedling growth, but RPL10A-overexpressing lines are more sensitive to ABA during early plant development and exhibit higher stomatal closure under light condition both with and without ABA treatment than wild type plants. Interestingly, RPL10A expression is induced by ABA. Together, we conclude that RPL10A could act as a positive regulator for ABA-dependent responses in Arabidopsis plants. Fil: Ramos, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Frontiers Media http://hdl.handle.net/11336/147342 Ramos, Rocío Soledad; Casati, Paula; Spampinato, Claudia Patricia; Falcone Ferreyra, María Lorena; Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis; Frontiers Media; Frontiers in Plant Science; 11; 11-2020; 1-14 1664-462X CONICET Digital CONICET |
| spellingShingle | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis Ramos, Rocío Soledad ABA, EARLY SEEDLING DEVELOPMENT RPL10A MUTANT RPL10A-OVEREXPRESSING LINES SEED GERMINATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis |
| title_full | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis |
| title_fullStr | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis |
| title_full_unstemmed | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis |
| title_short | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis |
| title_sort | Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis |
| topic | ABA, EARLY SEEDLING DEVELOPMENT RPL10A MUTANT RPL10A-OVEREXPRESSING LINES SEED GERMINATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | ABA, EARLY SEEDLING DEVELOPMENT RPL10A MUTANT RPL10A-OVEREXPRESSING LINES SEED GERMINATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/147342 |