Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
Ethnopharmacological relevance: Ulomoides dermestoides (Fairmaire, 1893) is a cosmopolitan tenebrionid beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment of different illnesses such as asthma, Parkinson's, diabetes, arthritis, HIV and specially cance...
| Authors: | ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2011 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/78831 |
| Online Access: | http://hdl.handle.net/11336/78831 |
| Access Level: | Open access |
| Keyword: | Benzoquinones Cytotoxicity Genotoxicity Pentadecene Tenebrionidae Ulomoides Dermestoides https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403178314072064 |
|---|---|
| author | Crespo, Rosana |
| author2 | Villaverde, Maria Luciana Girotti, Juan Roberto Güerci, Alba Mabel Juarez, Marta Patricia de Bravo, M.G. |
| author2_role | author author author author author |
| author_browse | Crespo, Rosana Girotti, Juan Roberto Güerci, Alba Mabel Juarez, Marta Patricia Villaverde, Maria Luciana de Bravo, M.G. |
| author_facet | Crespo, Rosana Villaverde, Maria Luciana Girotti, Juan Roberto Güerci, Alba Mabel Juarez, Marta Patricia de Bravo, M.G. |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Ethnopharmacological relevance: Ulomoides dermestoides (Fairmaire, 1893) is a cosmopolitan tenebrionid beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment of different illnesses such as asthma, Parkinson's, diabetes, arthritis, HIV and specially cancer. Aim of the study: To evaluate the cytotoxicity and DNA damage of the major volatile components released by Ulomoides dermestoides on human lung carcinoma epithelial cell line A549. Materials and methods: The defence compounds of Ulomoides dermestoides were extracted with dichloromethane and analyzed and quantified by capillary gas chromatography. The toxicity effects of the beetle's extract against A549 cell line were evaluated. Cytotoxicity was evaluated by MTT test and Trypan blue assay and genotoxicity was evaluated by the comet assay. The synthetic compounds, individually or combined, were also tested in A549 cells and normal mononuclear human cells. Results: The defence compounds of Ulomoides dermestoides extracted with dichloromethane (methyl-1,4-benzoquinones, ethyl-1,4-benzoquinones and 1-pentadecene as major components) showed cytotoxic activity on A549 cells demonstrated by MTT test and Trypan blue assay, with IC 50 values of 0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend (0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2 ± 2.7% and induced significant DNA damage both in tumor and mononuclear cells. Conclusion: Results of this study demonstrated that defence compounds of Ulomoides dermestoides reduced cell viability and induced DNA damage. We also concluded that the insect benzoquinones are primarily responsible for inducing cytotoxicity and genotoxicity in culture cells. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_4cfeeef7a99196cd48ccb60fe9dfd646 |
| identifier_str_mv | Crespo, Rosana; Villaverde, Maria Luciana; Girotti, Juan Roberto; Güerci, Alba Mabel; Juarez, Marta Patricia; et al.; Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells; Elsevier Ireland; Journal of Ethnopharmacology; 136; 1; 6-2011; 204-209 0378-8741 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/78831 |
| publishDate | 2011 |
| publishDateSort | 2011 |
| publisher | Elsevier Ireland |
| publisherStr | Elsevier Ireland |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells Crespo, Rosana Villaverde, Maria Luciana Girotti, Juan Roberto Güerci, Alba Mabel Juarez, Marta Patricia de Bravo, M.G. Benzoquinones Cytotoxicity Genotoxicity Pentadecene Tenebrionidae Ulomoides Dermestoides https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Ethnopharmacological relevance: Ulomoides dermestoides (Fairmaire, 1893) is a cosmopolitan tenebrionid beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment of different illnesses such as asthma, Parkinson's, diabetes, arthritis, HIV and specially cancer. Aim of the study: To evaluate the cytotoxicity and DNA damage of the major volatile components released by Ulomoides dermestoides on human lung carcinoma epithelial cell line A549. Materials and methods: The defence compounds of Ulomoides dermestoides were extracted with dichloromethane and analyzed and quantified by capillary gas chromatography. The toxicity effects of the beetle's extract against A549 cell line were evaluated. Cytotoxicity was evaluated by MTT test and Trypan blue assay and genotoxicity was evaluated by the comet assay. The synthetic compounds, individually or combined, were also tested in A549 cells and normal mononuclear human cells. Results: The defence compounds of Ulomoides dermestoides extracted with dichloromethane (methyl-1,4-benzoquinones, ethyl-1,4-benzoquinones and 1-pentadecene as major components) showed cytotoxic activity on A549 cells demonstrated by MTT test and Trypan blue assay, with IC 50 values of 0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend (0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2 ± 2.7% and induced significant DNA damage both in tumor and mononuclear cells. Conclusion: Results of this study demonstrated that defence compounds of Ulomoides dermestoides reduced cell viability and induced DNA damage. We also concluded that the insect benzoquinones are primarily responsible for inducing cytotoxicity and genotoxicity in culture cells. Fil: Crespo, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: Villaverde, Maria Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: Girotti, Juan Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Centro de Investigación de Transferencia en Oncología Molecular Argentina; Argentina Fil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: de Bravo, M.G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Elsevier Ireland http://hdl.handle.net/11336/78831 Crespo, Rosana; Villaverde, Maria Luciana; Girotti, Juan Roberto; Güerci, Alba Mabel; Juarez, Marta Patricia; et al.; Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells; Elsevier Ireland; Journal of Ethnopharmacology; 136; 1; 6-2011; 204-209 0378-8741 CONICET Digital CONICET |
| spellingShingle | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells Crespo, Rosana Benzoquinones Cytotoxicity Genotoxicity Pentadecene Tenebrionidae Ulomoides Dermestoides https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells |
| title_full | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells |
| title_fullStr | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells |
| title_full_unstemmed | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells |
| title_short | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells |
| title_sort | Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells |
| topic | Benzoquinones Cytotoxicity Genotoxicity Pentadecene Tenebrionidae Ulomoides Dermestoides https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | Benzoquinones Cytotoxicity Genotoxicity Pentadecene Tenebrionidae Ulomoides Dermestoides https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/78831 |