Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection
Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the...
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2015 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/144417 |
| Online Access: | http://hdl.handle.net/11336/144417 |
| Access Level: | Open access |
| Keyword: | CHAGAS DISEASE GENOME HEMATOPHAGY IMMUNITY RHODNIUS PROLIXUS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878402971103920128 |
|---|---|
| author | Calderón Fernández, Gustavo Mario |
| author2 | Esponda Behrens, Natalia Irene Juarez, Marta Patricia Latorre Estivalis, Jose Manuel Lavore, Andres Esteban Lazzari, Claudio Ricardo Lorenzo, Marcelo Gustavo Ons, Sheila Pagola, Lucia Elena Pascual, Agustina Pedrini, Nicolás Sterkel, Marcos |
| author2_role | author author author author author author author author author author author |
| author_browse | Calderón Fernández, Gustavo Mario Esponda Behrens, Natalia Irene Juarez, Marta Patricia Latorre Estivalis, Jose Manuel Lavore, Andres Esteban Lazzari, Claudio Ricardo Lorenzo, Marcelo Gustavo Ons, Sheila Pagola, Lucia Elena Pascual, Agustina Pedrini, Nicolás Sterkel, Marcos |
| author_facet | Calderón Fernández, Gustavo Mario Esponda Behrens, Natalia Irene Juarez, Marta Patricia Latorre Estivalis, Jose Manuel Lavore, Andres Esteban Lazzari, Claudio Ricardo Lorenzo, Marcelo Gustavo Ons, Sheila Pagola, Lucia Elena Pascual, Agustina Pedrini, Nicolás Sterkel, Marcos |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (?702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immunedeficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_254e4cdceb31c115b11d7d5b4e1afc19 |
| identifier_str_mv | Calderón Fernández, Gustavo Mario; Esponda Behrens, Natalia Irene; Juarez, Marta Patricia; Latorre Estivalis, Jose Manuel; Lavore, Andres Esteban; et al.; Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 112; 48; 16-11-2015; 14936-14941 0027-8424 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/144417 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection Calderón Fernández, Gustavo Mario Esponda Behrens, Natalia Irene Juarez, Marta Patricia Latorre Estivalis, Jose Manuel Lavore, Andres Esteban Lazzari, Claudio Ricardo Lorenzo, Marcelo Gustavo Ons, Sheila Pagola, Lucia Elena Pascual, Agustina Pedrini, Nicolás Sterkel, Marcos CHAGAS DISEASE GENOME HEMATOPHAGY IMMUNITY RHODNIUS PROLIXUS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (?702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immunedeficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods. Fil: Calderón Fernández, Gustavo Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: Esponda Behrens, Natalia Irene. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Oswaldo Cruz; Brasil. Instituto Nacional de Ciencia e Tecnologia en Entomología Molecular; Brasil Fil: Lavore, Andres Esteban. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Lazzari, Claudio Ricardo. Université François Rabelais; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lorenzo, Marcelo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Oswaldo Cruz; Brasil. Instituto Nacional de Ciencia e Tecnologia en Entomología Molecular; Brasil Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Pagola, Lucia Elena. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Pascual, Agustina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: Sterkel, Marcos. Universidade Federal do Rio de Janeiro; Brasil. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina National Academy of Sciences http://hdl.handle.net/11336/144417 Calderón Fernández, Gustavo Mario; Esponda Behrens, Natalia Irene; Juarez, Marta Patricia; Latorre Estivalis, Jose Manuel; Lavore, Andres Esteban; et al.; Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 112; 48; 16-11-2015; 14936-14941 0027-8424 CONICET Digital CONICET |
| spellingShingle | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection Calderón Fernández, Gustavo Mario CHAGAS DISEASE GENOME HEMATOPHAGY IMMUNITY RHODNIUS PROLIXUS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection |
| title_full | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection |
| title_fullStr | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection |
| title_full_unstemmed | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection |
| title_short | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection |
| title_sort | Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection |
| topic | CHAGAS DISEASE GENOME HEMATOPHAGY IMMUNITY RHODNIUS PROLIXUS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | CHAGAS DISEASE GENOME HEMATOPHAGY IMMUNITY RHODNIUS PROLIXUS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/144417 |