Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host
Fil: Naidich, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
| Authors: | ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2006 |
| Country: | Argentina |
| Institution: | ANLIS |
| Repository: | Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
| Language: | English |
| OAI Identifier: | oai:sgc.anlis.gob.ar:123456789/2225 |
| Online Access: | http://sgc.anlis.gob.ar/handle/123456789/2225 |
| Access Level: | Open access |
| Keyword: | Echinococcus granulosus Equinococosis Patente |
| _version_ | 1878402962742575104 |
|---|---|
| author | Naidich, Ariel |
| author2 | McManus, Donald P Canova, S G Gutierrez, Ariana M. Zhang, Wenbao Guarnera, Eduardo Rosenzvit, Mara Cecilia |
| author2_role | author author author author author author |
| author_browse | Canova, S G Guarnera, Eduardo Gutierrez, Ariana M. McManus, Donald P Naidich, Ariel Rosenzvit, Mara Cecilia Zhang, Wenbao |
| author_facet | Naidich, Ariel McManus, Donald P Canova, S G Gutierrez, Ariana M. Zhang, Wenbao Guarnera, Eduardo Rosenzvit, Mara Cecilia |
| author_role | author |
| collection | Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
| description | Fil: Naidich, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_23821cb1663f5a5d858f7aa07fe747dc |
| identifier_str_mv | 0890-8508 10.1016/j.mcp.2005.08.001 |
| institution | ANLIS |
| instname_str | Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:sgc.anlis.gob.ar:123456789/2225 |
| publishDate | 2006 |
| publishDateSort | 2006 |
| reponame_str | Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
| spelling | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host Naidich, Ariel McManus, Donald P Canova, S G Gutierrez, Ariana M. Zhang, Wenbao Guarnera, Eduardo Rosenzvit, Mara Cecilia Echinococcus granulosus Equinococosis Patente Fil: Naidich, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: McManus, Donald P. Royal Brisbane Hospital Post Office. Queensland Institute of Medical Research. Molecular Parasitology Laboratory. Division of Infectious Diseases and Immunology; Australia. Fil: Canova, Sergio G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Gutierrez, Ariana M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Zhang, Wenbao. Royal Brisbane Hospital Post Office. Queensland Institute of Medical Research. Molecular Parasitology Laboratory. Division of Infectious Diseases and Immunology; Australia. Fil: Guarnera, Eduardo A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Rosenzvit, Mara Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. The detection of Echinococcus granulosus in dogs is important for epidemiological surveillance and evaluation of cystic hydatic disease control programs. We report the efficacy of two PCR-based methods to detect patent and pre-patent infection in dogs experimentally infected with E. granulosus. The detection is based on amplification of a fragment of a mitochondrial gene (Mit-PCR) and a DNA repetitive element (Rep-PCR) of E. granulosus. We tested the ability of both methods to detect several genotypes of the parasite. Both PCR methods could detect E. granulosus in pre-patent and patent periods, even when microscopical observation of eggs resulted negative in fecal samples. The Mit-PCR produced the same amplification pattern for all the parasite genotypes tested while the amplification patterns with the Rep-PCR differed among groups of strains. Fecal samples collected from dogs of an endemic area were diagnosed with more sensitivity than arecoline hydrobromide purgation. These molecular methods could be applied in the confirmation of coproantigen-positive fecal samples and to verify the success of control programs. 0890-8508 http://sgc.anlis.gob.ar/handle/123456789/2225 10.1016/j.mcp.2005.08.001 |
| spellingShingle | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host Naidich, Ariel Echinococcus granulosus Equinococosis Patente |
| status_str | publishedVersion |
| title | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host |
| title_full | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host |
| title_fullStr | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host |
| title_full_unstemmed | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host |
| title_short | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host |
| title_sort | Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host |
| topic | Echinococcus granulosus Equinococosis Patente |
| topic_facet | Echinococcus granulosus Equinococosis Patente |
| url | http://sgc.anlis.gob.ar/handle/123456789/2225 |