GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock
GIGANTEA (GI) is a nuclear protein involved in the promotion of flowering by long days, in light input to the circadian clock, and in seedling photomorphogenesis under continuous red light but not far-red light (FR). Here, we report that in Arabidopsis ( Arabidopsis thaliana ) different alleles of g...
| Authors: | ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2007 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/31050 |
| Online Access: | http://hdl.handle.net/11336/31050 |
| Access Level: | Open access |
| Keyword: | Flowering Photomorphogenesis Arabidopsis Thaliana https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878402850936061952 |
|---|---|
| author | Oliveiro, Karina Andrea |
| author2 | Crepy, Maria Andrea Martin Tryon, Ellen L. Milich, Raechel Harmer, Stacey L. Putterill, Jo Yanovsky, Marcelo Javier Casal, Jorge José |
| author2_role | author author author author author author author |
| author_browse | Casal, Jorge José Crepy, Maria Andrea Harmer, Stacey L. Martin Tryon, Ellen L. Milich, Raechel Oliveiro, Karina Andrea Putterill, Jo Yanovsky, Marcelo Javier |
| author_facet | Oliveiro, Karina Andrea Crepy, Maria Andrea Martin Tryon, Ellen L. Milich, Raechel Harmer, Stacey L. Putterill, Jo Yanovsky, Marcelo Javier Casal, Jorge José |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | GIGANTEA (GI) is a nuclear protein involved in the promotion of flowering by long days, in light input to the circadian clock, and in seedling photomorphogenesis under continuous red light but not far-red light (FR). Here, we report that in Arabidopsis ( Arabidopsis thaliana ) different alleles of gi have defects in the hypocotyl-growth and cotyledon-unfolding responses to hourly pulses of FR, a treatment perceived by phytochrome A (phyA). This phenotype is rescued by overexpression of GI. The very- low-fluence response of seed germination was also reduced in gi . Since the circadian clock modulates many light responses, we investigated whether these gi phenotypes were due to alterations in the circadian system or light signaling per se. In exper- iments where FR pulses were given to dark-incubated seeds or seedlings at different times of the day, gi showed reduced seed germination, cotyledon unfolding, and activity of a luciferase reporter fused to the promoter of a chlorophyll a/b -binding protein gene; however, rhythmic sensitivity was normal in these plants. We conclude that while GI does not affect the high- irradiance responses of phyA, it does affect phyA-mediated very-low-fluence responses via mechanisms that do not obviously involve its circadian functions |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_0e42ba319a5259a1ca0c474556c8a1cb |
| identifier_str_mv | Casal, Jorge José; Yanovsky, Marcelo Javier; Putterill, Jo; Harmer, Stacey L.; Oliveiro, Karina Andrea; Milich, Raechel; et al.; GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock; American Society of Plant Biologist; Plant Physiology; 144; 1; 3-2007; 495-502 0032-0889 1532-2548 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/31050 |
| publishDate | 2007 |
| publishDateSort | 2007 |
| publisher | American Society of Plant Biologist |
| publisherStr | American Society of Plant Biologist |
| reponame_str | CONICET Digital (CONICET) |
| spelling | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock Oliveiro, Karina Andrea Crepy, Maria Andrea Martin Tryon, Ellen L. Milich, Raechel Harmer, Stacey L. Putterill, Jo Yanovsky, Marcelo Javier Casal, Jorge José Flowering Photomorphogenesis Arabidopsis Thaliana https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 GIGANTEA (GI) is a nuclear protein involved in the promotion of flowering by long days, in light input to the circadian clock, and in seedling photomorphogenesis under continuous red light but not far-red light (FR). Here, we report that in Arabidopsis ( Arabidopsis thaliana ) different alleles of gi have defects in the hypocotyl-growth and cotyledon-unfolding responses to hourly pulses of FR, a treatment perceived by phytochrome A (phyA). This phenotype is rescued by overexpression of GI. The very- low-fluence response of seed germination was also reduced in gi . Since the circadian clock modulates many light responses, we investigated whether these gi phenotypes were due to alterations in the circadian system or light signaling per se. In exper- iments where FR pulses were given to dark-incubated seeds or seedlings at different times of the day, gi showed reduced seed germination, cotyledon unfolding, and activity of a luciferase reporter fused to the promoter of a chlorophyll a/b -binding protein gene; however, rhythmic sensitivity was normal in these plants. We conclude that while GI does not affect the high- irradiance responses of phyA, it does affect phyA-mediated very-low-fluence responses via mechanisms that do not obviously involve its circadian functions Fil: Oliveiro, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Crepy, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Martin Tryon, Ellen L.. University of California; Estados Unidos Fil: Milich, Raechel. The University Of Auckland; Nueva Zelanda Fil: Harmer, Stacey L.. University of California; Estados Unidos Fil: Putterill, Jo. The University Of Auckland; Nueva Zelanda Fil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina American Society of Plant Biologist http://hdl.handle.net/11336/31050 Casal, Jorge José; Yanovsky, Marcelo Javier; Putterill, Jo; Harmer, Stacey L.; Oliveiro, Karina Andrea; Milich, Raechel; et al.; GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock; American Society of Plant Biologist; Plant Physiology; 144; 1; 3-2007; 495-502 0032-0889 1532-2548 CONICET Digital CONICET |
| spellingShingle | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock Oliveiro, Karina Andrea Flowering Photomorphogenesis Arabidopsis Thaliana https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock |
| title_full | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock |
| title_fullStr | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock |
| title_full_unstemmed | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock |
| title_short | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock |
| title_sort | GIGANTEA regulates phytochrome A-mediated photomorphogenesis independently of its role in the circadian clock |
| topic | Flowering Photomorphogenesis Arabidopsis Thaliana https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | Flowering Photomorphogenesis Arabidopsis Thaliana https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/31050 |