Involvement of SARA in axon and dendrite growth
SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis in cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the role of SARA during neuronal development and tested whether it functions as a regulator of endocy...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2015 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/69401 |
| Online Access: | http://hdl.handle.net/11336/69401 |
| Access Level: | Open access |
| Keyword: | AXON DENDRITE POLARITY ENDOSOMES https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878402780904816640 |
|---|---|
| author | Arias, Cristina Isabel |
| author2 | Siri, Sebastian Omar Conde, Cecilia Beatriz |
| author2_role | author author |
| author_browse | Arias, Cristina Isabel Conde, Cecilia Beatriz Siri, Sebastian Omar |
| author_facet | Arias, Cristina Isabel Siri, Sebastian Omar Conde, Cecilia Beatriz |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis in cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the role of SARA during neuronal development and tested whether it functions as a regulator of endocytic trafficking of selected axonal and membrane proteins. Suppression of SARA perturbs the appearance of juxtanuclear endocytic recycling compartments and the neurons show long axons with large growth cones. Furthermore, surface distribution of the cell adhesion molecule L1 in axons and the fusion of vesicles containing transferring receptor (TfR) in dendrites were increased in neurons where SARA was silenced. Conversely, SARA overexpression generated large early endosomes and reduced neurite outgrowth. Taken together, our findings suggest a significant contribution of SARA to key aspects of neuronal development, including neurite formation. Copyright: |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_02f4d4cd36a65fe8a8d7e1f8675bcf2e |
| identifier_str_mv | Arias, Cristina Isabel; Siri, Sebastian Omar; Conde, Cecilia Beatriz; Involvement of SARA in axon and dendrite growth; Public Library of Science; Plos One; 10; 9; 9-2015; 1-19 1932-6203 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/69401 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Involvement of SARA in axon and dendrite growth Arias, Cristina Isabel Siri, Sebastian Omar Conde, Cecilia Beatriz AXON DENDRITE POLARITY ENDOSOMES https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis in cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the role of SARA during neuronal development and tested whether it functions as a regulator of endocytic trafficking of selected axonal and membrane proteins. Suppression of SARA perturbs the appearance of juxtanuclear endocytic recycling compartments and the neurons show long axons with large growth cones. Furthermore, surface distribution of the cell adhesion molecule L1 in axons and the fusion of vesicles containing transferring receptor (TfR) in dendrites were increased in neurons where SARA was silenced. Conversely, SARA overexpression generated large early endosomes and reduced neurite outgrowth. Taken together, our findings suggest a significant contribution of SARA to key aspects of neuronal development, including neurite formation. Copyright: Fil: Arias, Cristina Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Siri, Sebastian Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Conde, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Public Library of Science http://hdl.handle.net/11336/69401 Arias, Cristina Isabel; Siri, Sebastian Omar; Conde, Cecilia Beatriz; Involvement of SARA in axon and dendrite growth; Public Library of Science; Plos One; 10; 9; 9-2015; 1-19 1932-6203 CONICET Digital CONICET |
| spellingShingle | Involvement of SARA in axon and dendrite growth Arias, Cristina Isabel AXON DENDRITE POLARITY ENDOSOMES https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Involvement of SARA in axon and dendrite growth |
| title_full | Involvement of SARA in axon and dendrite growth |
| title_fullStr | Involvement of SARA in axon and dendrite growth |
| title_full_unstemmed | Involvement of SARA in axon and dendrite growth |
| title_short | Involvement of SARA in axon and dendrite growth |
| title_sort | Involvement of SARA in axon and dendrite growth |
| topic | AXON DENDRITE POLARITY ENDOSOMES https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | AXON DENDRITE POLARITY ENDOSOMES https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/69401 |