Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography
The quantitative determination of boldine alkaloid in boldo leaf extracts by employing cyclic voltammetry, at a liquid/liquid interface as well as the validation of this methodology against the reference method, high performance liquid chromatography (HPLC), are reported in the present paper. The vo...
| Authors: | ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2010 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/69832 |
| Online Access: | http://hdl.handle.net/11336/69832 |
| Access Level: | Open access |
| Keyword: | Acid Dissociation Constant Boldine Alkaloids Electrochemistry at Liquid/Liquid Interfaces Quantification of Complex Matrixes Validation of Electrochemical Method https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| _version_ | 1878402763982897152 |
|---|---|
| author | Cámara, Candelaria Inés |
| author2 | Bornancini, Carlos Alberto Cabrera, Jose Luis Ortega, María Gabriela Yudi, Lidia Mabel |
| author2_role | author author author author |
| author_browse | Bornancini, Carlos Alberto Cabrera, Jose Luis Cámara, Candelaria Inés Ortega, María Gabriela Yudi, Lidia Mabel |
| author_facet | Cámara, Candelaria Inés Bornancini, Carlos Alberto Cabrera, Jose Luis Ortega, María Gabriela Yudi, Lidia Mabel |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | The quantitative determination of boldine alkaloid in boldo leaf extracts by employing cyclic voltammetry, at a liquid/liquid interface as well as the validation of this methodology against the reference method, high performance liquid chromatography (HPLC), are reported in the present paper. The voltammetric analysis was performed successfully and economically using two kinds of liquid/liquid interfaces: water/1,2-dicholoroethane and water/PVC (polyvinyl chloride)-gelled 1,2-dichloroethane. Linear calibration curves in the concentration range of 1.04 × 10-5 mol L-1 to 5.19 × 10-4 mol L-1 were obtained with a detection limit equal to (6.1 ± 0.7) × 10-5 mol L-1 and the quantitative determination of this alkaloid, in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was found to be equal to the values obtained using the standard HPLC method. The validation analysis of this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed is economical and selective, involves simple equipment and can be applied for the quantitative determination of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation. Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value of pKa w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_00aec0fbfbb7e2aa387e1ba14262f94a |
| identifier_str_mv | Cámara, Candelaria Inés; Bornancini, Carlos Alberto; Cabrera, Jose Luis; Ortega, María Gabriela; Yudi, Lidia Mabel; Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography; Elsevier Science; Talanta; 83; 2; 12-2010; 623-630 0039-9140 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/69832 |
| publishDate | 2010 |
| publishDateSort | 2010 |
| publisher | Elsevier Science |
| publisherStr | Elsevier Science |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography Cámara, Candelaria Inés Bornancini, Carlos Alberto Cabrera, Jose Luis Ortega, María Gabriela Yudi, Lidia Mabel Acid Dissociation Constant Boldine Alkaloids Electrochemistry at Liquid/Liquid Interfaces Quantification of Complex Matrixes Validation of Electrochemical Method https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 The quantitative determination of boldine alkaloid in boldo leaf extracts by employing cyclic voltammetry, at a liquid/liquid interface as well as the validation of this methodology against the reference method, high performance liquid chromatography (HPLC), are reported in the present paper. The voltammetric analysis was performed successfully and economically using two kinds of liquid/liquid interfaces: water/1,2-dicholoroethane and water/PVC (polyvinyl chloride)-gelled 1,2-dichloroethane. Linear calibration curves in the concentration range of 1.04 × 10-5 mol L-1 to 5.19 × 10-4 mol L-1 were obtained with a detection limit equal to (6.1 ± 0.7) × 10-5 mol L-1 and the quantitative determination of this alkaloid, in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was found to be equal to the values obtained using the standard HPLC method. The validation analysis of this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed is economical and selective, involves simple equipment and can be applied for the quantitative determination of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation. Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value of pKa w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH. Fil: Cámara, Candelaria Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina Fil: Bornancini, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Yudi, Lidia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina Elsevier Science http://hdl.handle.net/11336/69832 Cámara, Candelaria Inés; Bornancini, Carlos Alberto; Cabrera, Jose Luis; Ortega, María Gabriela; Yudi, Lidia Mabel; Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography; Elsevier Science; Talanta; 83; 2; 12-2010; 623-630 0039-9140 CONICET Digital CONICET |
| spellingShingle | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography Cámara, Candelaria Inés Acid Dissociation Constant Boldine Alkaloids Electrochemistry at Liquid/Liquid Interfaces Quantification of Complex Matrixes Validation of Electrochemical Method https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography |
| title_full | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography |
| title_fullStr | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography |
| title_full_unstemmed | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography |
| title_short | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography |
| title_sort | Quantitative analysis of boldine alkaloid in natural extracts by cyclic voltammetry at a liquid-liquid interface and validation of the method by comparison with high performance liquid chromatography |
| topic | Acid Dissociation Constant Boldine Alkaloids Electrochemistry at Liquid/Liquid Interfaces Quantification of Complex Matrixes Validation of Electrochemical Method https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| topic_facet | Acid Dissociation Constant Boldine Alkaloids Electrochemistry at Liquid/Liquid Interfaces Quantification of Complex Matrixes Validation of Electrochemical Method https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/69832 |