SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors
Chronic lymphocytic leukemia (CLL) is characterized by a highly variable clinical course. Microarray studies allowed highlight genes differentially expressed in this pathology. In this study, we have evaluated the prognostic significance of SEPT10 expression in CLL patients. Results were correlated...
| Authors: | ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2013 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/26820 |
| Online Access: | http://hdl.handle.net/11336/26820 |
| Access Level: | Open access |
| Keyword: | CHRONIC LYMPHOCYTIC LEUKEMIA GENE EXPRESSION SEPT10 IGHV https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| _version_ | 1878402763905302528 |
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| author | Travella, Ana Carolina |
| author2 | Panero, Julieta Stanganelli, Carmen Graciela Bezares, Raimundo F. Slavutsky, Irma Rosa |
| author2_role | author author author author |
| author_browse | Bezares, Raimundo F. Panero, Julieta Slavutsky, Irma Rosa Stanganelli, Carmen Graciela Travella, Ana Carolina |
| author_facet | Travella, Ana Carolina Panero, Julieta Stanganelli, Carmen Graciela Bezares, Raimundo F. Slavutsky, Irma Rosa |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | Chronic lymphocytic leukemia (CLL) is characterized by a highly variable clinical course. Microarray studies allowed highlight genes differentially expressed in this pathology. In this study, we have evaluated the prognostic significance of SEPT10 expression in CLL patients. Results were correlated with immunoglobulin heavy-chain variable (IGHV) genes mutational status, genomic rearrangements and clinical parameters. SEPT10 mRNA levels were determined by quantitative real-time PCR in 70 newly diagnosed CLL patients consecutively referred to our Institution. A wide heterogeneity for SEPT10 expression was found. Gene upregulation was observed in 18.5% of cases. The univariate analysis showed a positive association between gen expression and platelet count (p < 0.0001) and a negative correlation with hemoglobin levels (p = 0.0094). Although no significant differences were observed, mean treatment free survival was shorter in patients with high expression (31 months) with respect to those with low mRNA levels (72 months). Cases with abnormal karyotypes had increased expression compared to those with normal karyotypes and no association between gene expression and FISH (fluorescence in situ hybridization) risk groups and IGHV mutational status was found. Cases using IGHV3-23 gene rearrangement had low SEPT10 expression. Our results showed an association between SEPT10 expression and features of adverse outcome but without independent prognostic value. The study of SEPT10 expression may be important for a better understanding of disease heterogeneity, adding further information to those provided by established prognostic factors. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_00a48c6b7ee32493a79e77ffacd5e706 |
| identifier_str_mv | Travella, Ana Carolina; Panero, Julieta; Stanganelli, Carmen Graciela; Bezares, Raimundo F.; Slavutsky, Irma Rosa; SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors; Scientific Research; Open Journal of Blood Diseases; 3; 3A; 10-2013; 11-16 2164-3199 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/26820 |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher | Scientific Research |
| publisherStr | Scientific Research |
| reponame_str | CONICET Digital (CONICET) |
| spelling | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors Travella, Ana Carolina Panero, Julieta Stanganelli, Carmen Graciela Bezares, Raimundo F. Slavutsky, Irma Rosa CHRONIC LYMPHOCYTIC LEUKEMIA GENE EXPRESSION SEPT10 IGHV https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 Chronic lymphocytic leukemia (CLL) is characterized by a highly variable clinical course. Microarray studies allowed highlight genes differentially expressed in this pathology. In this study, we have evaluated the prognostic significance of SEPT10 expression in CLL patients. Results were correlated with immunoglobulin heavy-chain variable (IGHV) genes mutational status, genomic rearrangements and clinical parameters. SEPT10 mRNA levels were determined by quantitative real-time PCR in 70 newly diagnosed CLL patients consecutively referred to our Institution. A wide heterogeneity for SEPT10 expression was found. Gene upregulation was observed in 18.5% of cases. The univariate analysis showed a positive association between gen expression and platelet count (p < 0.0001) and a negative correlation with hemoglobin levels (p = 0.0094). Although no significant differences were observed, mean treatment free survival was shorter in patients with high expression (31 months) with respect to those with low mRNA levels (72 months). Cases with abnormal karyotypes had increased expression compared to those with normal karyotypes and no association between gene expression and FISH (fluorescence in situ hybridization) risk groups and IGHV mutational status was found. Cases using IGHV3-23 gene rearrangement had low SEPT10 expression. Our results showed an association between SEPT10 expression and features of adverse outcome but without independent prognostic value. The study of SEPT10 expression may be important for a better understanding of disease heterogeneity, adding further information to those provided by established prognostic factors. Fil: Travella, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina Fil: Stanganelli, Carmen Graciela. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina Fil: Bezares, Raimundo F.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Dr. Teodoro Álvarez"; Argentina Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina Scientific Research http://hdl.handle.net/11336/26820 Travella, Ana Carolina; Panero, Julieta; Stanganelli, Carmen Graciela; Bezares, Raimundo F.; Slavutsky, Irma Rosa; SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors; Scientific Research; Open Journal of Blood Diseases; 3; 3A; 10-2013; 11-16 2164-3199 CONICET Digital CONICET |
| spellingShingle | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors Travella, Ana Carolina CHRONIC LYMPHOCYTIC LEUKEMIA GENE EXPRESSION SEPT10 IGHV https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| status_str | publishedVersion |
| title | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors |
| title_full | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors |
| title_fullStr | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors |
| title_full_unstemmed | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors |
| title_short | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors |
| title_sort | SEPT10 expression in chronic lymphocytic leukemia. Correlation with clinical and biological prognostic factors |
| topic | CHRONIC LYMPHOCYTIC LEUKEMIA GENE EXPRESSION SEPT10 IGHV https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| topic_facet | CHRONIC LYMPHOCYTIC LEUKEMIA GENE EXPRESSION SEPT10 IGHV https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| url | http://hdl.handle.net/11336/26820 |