Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C280 and EspB proteins

Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This stud...

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Autores: Vilte, Daniel A.; Larzabal, Mariano; Garbaccio, Sergio; Gammella, Mariela; Rabinovitz, Bettina Carol; Elizondo, Ana M.; Cantet, Rodolfo Juan Carlos; Delgado, Fernando; Meikle, Virginia; Cataldi, Angel Adrian; Mercado, Elsa C.
Tipo de documento: Artigo
Estado:Published version
Data de publicação:2011
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:Inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/16294
Acesso em linha:http://hdl.handle.net/11336/16294
Nível de acesso:Acesso aberto
Palavra-chave:E.Coli O157:H7
Cattle
Vaccination
Intimin Espb
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
Resumo:Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of -intimin (-intimin C280) and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle.

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