Petroleum hydrocarbon degradation by isolated mangrove bacteria

The petroleum hydrocarbon contamination represents a worldwide problem, since its accumulation promotes a serious environmental impact. Thereby, the use of microorganisms, such as those from mangrove micro biota, as degrading agents of various carbon sources is poorly exploited in environmental reme...

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Detalhes bibliográficos
Autores: Lustosa, Mayara A.; López, Jorge A.; Santos Freire, Karla C.; Padilha, Francine F.; Hernández-Macedo, María Lucila; Cabrera-Padilla, Rebeca Y.
Tipo de documento: Artigo
Estado:Published version
Data de publicação:2018
País:Perú
Recursos:UNMSM
Repositório:Revistas - Universidad Nacional Mayor de San Marcos
Idioma:Inglês
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/15537
Acesso em linha:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/15537
Nível de acesso:Acesso aberto
Palavra-chave:microorganisms
metabolism
Hydrocarbons
pollution
bioremediation.
microorganismos
metabolismo
hidrocarburos
contaminación
biorremediación.
Descrição
Resumo:The petroleum hydrocarbon contamination represents a worldwide problem, since its accumulation promotes a serious environmental impact. Thereby, the use of microorganisms, such as those from mangrove micro biota, as degrading agents of various carbon sources is poorly exploited in environmental remediation processes. Thus, this in vitro study evaluated the degrading potential of isolated bacteria from mangrove sediments in the degradation of petroleum hydrocarbons. Analysis of the genetic diversity using the 16S rRNA marker revealed closely related (99%) sequences with Proteobacterium, Pseudomonas and Exiguobacterium. Results showed the bacterial growth in the mineral saline medium (MSM) containing 1% petroleum or diesel, as carbon sources. This growth was determinated by optical density at 595 nm for 15 days, with sample withdrawal every 48 h. Bacterial growth indicated the hydrocarbon metabolization. However, bacteria were more efficient at degrading petroleum. Overall, experimental data displayed the potential application of these bacteria in bioremediation processes, due to their metabolic and adaptive capacities to grow in a rich hydrocarbon medium.