Plasma gradient modified scaffolds to generate a chemoattractant surface

A gradient of plasma polymerization of pyrrole was generate d to model a chemoattractant surface; it was obtained by changing the polymerization tim e to manipulate the thickness and the deposite d layer. The polymerization was generated with a power of 30W on a cylindrical vac uum reactor, the cove...

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Bibliographic Details
Authors: O. Ramírez-Fernández; R. Godínez; E. Zúñiga-Aguilar; C. A. López-Cobá; J. Morales; R. Olayo
Format: Article
Status:Published version
Publication date:2014
Country:México
Institution:UAM
Repository:Redalyc-UAM
OAI Identifier:oai:redalyc.org:94231175005
Online Access:https://www.redalyc.org/articulo.oa?id=94231175005
Access Level:Open access
Keyword:Física, Astronomía y Matemáticas
Ito cells
Chemotaxis
Cell Culture
Biomaterials
Chemoattractant
Description
Summary:A gradient of plasma polymerization of pyrrole was generate d to model a chemoattractant surface; it was obtained by changing the polymerization tim e to manipulate the thickness and the deposite d layer. The polymerization was generated with a power of 30W on a cylindrical vac uum reactor, the coverslip was polymerized covered by a cavity with a surface exposed of 0.7 cm every 35 min., the cells seeded were liver stellate cells (Ito); it was evaluated against a coverslip with continuous plasma polymerization of pyrrole (Ppy). The results show similar protein secret ions, but cells proliferation was selective in the surface gradient regions.