The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation

The S:A222V point mutation, within the G clade, was characteristic of the 20E (EU1) SARS-CoV-2 variant identified in Spain in early summer 2020. This mutation has since reappeared in the Delta subvariant AY.4.2, raising questions about its specific effect on viral infection. We report combined serol...

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Authors: Ginex, Tiziana; Marco Marín, Clara; Wieczór, Miłosz; Mata, Carlos P.; Krieger, James; Ruiz Rodriguez, Paula; López Redondo, Maria Luisa; Francés Gómez, Clara; Melero, Roberto; Sánchez Sorzano, Carlos Óscar; Martínez, Marta; Gougeard, Nadine; Forcada Nadal, Alicia; Zamora Caballero, Sara; Gozalbo Rovira, Roberto; Sanz Frasquet, Carla; Arranz, Rocío; Bravo, Jeronimo; Rubio, Vicente; Marina, Alberto; Geller, Ron; Comas, Iñaki; Gil, Carmen; Coscolla, Mireia; Orozco López, Modesto; Llácer, José Luis; Carazo, Jose Maria; The IBV Covid19 Pipeline
Format: Article
Status:Published version
Publication date:2022
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/189320
Online Access:https://hdl.handle.net/2445/189320
Access Level:Open access
Keyword:SARS-CoV-2
Mutació (Biologia)
Mutation (Biology)
Summary:The S:A222V point mutation, within the G clade, was characteristic of the 20E (EU1) SARS-CoV-2 variant identified in Spain in early summer 2020. This mutation has since reappeared in the Delta subvariant AY.4.2, raising questions about its specific effect on viral infection. We report combined serological, functional, structural and computational studies characterizing the impact of this mutation. Our results reveal that S:A222V promotes an increased RBD opening and slightly increases ACE2 binding as compared to the parent S:D614G clade. Finally, S:A222V does not reduce sera neutralization capacity, suggesting it does not affect vaccine effectiveness.

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