Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
In this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2) is reported. It involves the use of sandwich-type immunoassays using selective capture and detector antibodies and carboxy...
| Autores: | ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/23869 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/23869 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Ara h 1 Ara h 2 dual determination magnetic beads SPdCEs amperometric immunosensor food extracts Química analítica (Química) 2301 Química Analítica |
| _version_ | 1878732171523391488 |
|---|---|
| author | Ruiz Valdepeñas Montiel, Víctor |
| author2 | Torrente Rodríguez, Rebeca Magnolia Campuzano Ruiz, Susana Pellicanò, Alessandro Reviejo García, Ángel Julio Cosio, Maria Pingarrón Carrazón, José Manuel |
| author2_role | author author author author author author |
| author_browse | Campuzano Ruiz, Susana Cosio, Maria Pellicanò, Alessandro Pingarrón Carrazón, José Manuel Reviejo García, Ángel Julio Ruiz Valdepeñas Montiel, Víctor Torrente Rodríguez, Rebeca Magnolia |
| author_facet | Ruiz Valdepeñas Montiel, Víctor Torrente Rodríguez, Rebeca Magnolia Campuzano Ruiz, Susana Pellicanò, Alessandro Reviejo García, Ángel Julio Cosio, Maria Pingarrón Carrazón, José Manuel |
| author_role | author |
| collection | Docta Complutense |
| description | In this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2) is reported. It involves the use of sandwich-type immunoassays using selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Amperometric detection at −0.20 V was performed using dual screen-printed carbon electrodes (SPdCEs) and the H2O2/hydroquinone (HQ) system. This methodology exhibits high sensitivity and selectivity for the target proteins providing detection limits of 18.0 and 0.07 ng/mL for Ara h 1 and Ara h 2, respectively, with an assay time of only 2 h. The usefulness of the approach was evaluated by detecting the endogenous content of both allergenic proteins in different food extracts as well as trace amounts of peanut allergen (0.0001% or 1.0 mg/kg) in wheat flour spiked samples. The developed platform provides better Low detection limits (LODs) in shorter assay times than those claimed for the allergen specific commercial ELISA kits using the same immunoreagents and quantitative information on individual food allergen levels. Moreover, the flexibility of the methodology makes it readily translate to the detection of other food-allergens. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_3dea4e8883bf86a47c55d0fbf0ca445a |
| institution | Universidad Complutense de Madrid (UCM) |
| instname_str | Universidad Complutense de Madrid (UCM) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:docta.ucm.es:20.500.14352/23869 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Docta Complutense |
| spelling | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms Ruiz Valdepeñas Montiel, Víctor Torrente Rodríguez, Rebeca Magnolia Campuzano Ruiz, Susana Pellicanò, Alessandro Reviejo García, Ángel Julio Cosio, Maria Pingarrón Carrazón, José Manuel Ara h 1 Ara h 2 dual determination magnetic beads SPdCEs amperometric immunosensor food extracts Química analítica (Química) 2301 Química Analítica In this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2) is reported. It involves the use of sandwich-type immunoassays using selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Amperometric detection at −0.20 V was performed using dual screen-printed carbon electrodes (SPdCEs) and the H2O2/hydroquinone (HQ) system. This methodology exhibits high sensitivity and selectivity for the target proteins providing detection limits of 18.0 and 0.07 ng/mL for Ara h 1 and Ara h 2, respectively, with an assay time of only 2 h. The usefulness of the approach was evaluated by detecting the endogenous content of both allergenic proteins in different food extracts as well as trace amounts of peanut allergen (0.0001% or 1.0 mg/kg) in wheat flour spiked samples. The developed platform provides better Low detection limits (LODs) in shorter assay times than those claimed for the allergen specific commercial ELISA kits using the same immunoreagents and quantitative information on individual food allergen levels. Moreover, the flexibility of the methodology makes it readily translate to the detection of other food-allergens. MDPI https://hdl.handle.net/20.500.14352/23869 |
| spellingShingle | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms Ruiz Valdepeñas Montiel, Víctor Ara h 1 Ara h 2 dual determination magnetic beads SPdCEs amperometric immunosensor food extracts Química analítica (Química) 2301 Química Analítica |
| title | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms |
| title_full | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms |
| title_fullStr | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms |
| title_full_unstemmed | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms |
| title_short | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms |
| title_sort | Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms |
| topic | Ara h 1 Ara h 2 dual determination magnetic beads SPdCEs amperometric immunosensor food extracts Química analítica (Química) 2301 Química Analítica |
| topic_facet | Ara h 1 Ara h 2 dual determination magnetic beads SPdCEs amperometric immunosensor food extracts Química analítica (Química) 2301 Química Analítica |
| url | https://hdl.handle.net/20.500.14352/23869 |