Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
In murine macrophages, as a result of arginine catabolism during activation, citruline isproduced under the effect of IFN-c and LPS, and ornithine and polyamines by IL-4 and IL-10. For proliferation, arginine is required from the extracellular medium and is used for protein synthesis. During activat...
| Autores: | ; ; ; ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Accepted version |
| Fecha de publicación: | 2006 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/206968 |
| Acceso en línea: | https://hdl.handle.net/2445/206968 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Macròfags Proliferació cel·lular Macrophages Cell proliferation |
| _version_ | 1878731408858415104 |
|---|---|
| author | Yeramian, Andrée |
| author2 | Martin, Lorena Arpa, Luis Bertran, Joan, 1964- Soler Prat, Concepció McLeod, Carol Modolell, Manuel Palacín Prieto, Manuel Lloberas Cavero, Jorge Celada Cotarelo, Antonio |
| author2_role | author author author author author author author author author |
| author_browse | Arpa, Luis Bertran, Joan, 1964- Celada Cotarelo, Antonio Lloberas Cavero, Jorge Martin, Lorena McLeod, Carol Modolell, Manuel Palacín Prieto, Manuel Soler Prat, Concepció Yeramian, Andrée |
| author_facet | Yeramian, Andrée Martin, Lorena Arpa, Luis Bertran, Joan, 1964- Soler Prat, Concepció McLeod, Carol Modolell, Manuel Palacín Prieto, Manuel Lloberas Cavero, Jorge Celada Cotarelo, Antonio |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | In murine macrophages, as a result of arginine catabolism during activation, citruline isproduced under the effect of IFN-c and LPS, and ornithine and polyamines by IL-4 and IL-10. For proliferation, arginine is required from the extracellular medium and is used for protein synthesis. During activation, most arginine (>95% in 6 h) was metabolized, while under proliferation only half was incorporated into proteins. Under basal conditions, this amino acid was preferentially transported by y+L activity. During activation, arginine transport increased drastically (4–5-fold) through y+ cationic amino acid transporter (CAT) activity. By contrast, M-CSF induced only a modest increase in uptake (0.5-fold). The increase in arginine transport during activation, but not proliferation, was mediated by the SLC7A2/Cat2 gene. SLC7A1/Cat1 is constitutively expressed, and is not modified by proliferating or activating agents.M-CSF-dependent proliferation was not affected in the macrophages of SLC7A2 knockout mice; however, these cells showed a drastic reduction in the production of citruline or ornithine and polyamines during activation. The data show that a large increase in a specific transport system (CAT2) is necessary for activation-induced arginine metabolism, while arginine is in excess for the requirements of proliferation and a modest increase in transport occurs. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_2f00a5cb3210a5a05d7dff964ae3ca9b |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/206968 |
| publishDate | 2006 |
| publishDateSort | 2006 |
| publisher | Wiley-VCH |
| publisherStr | Wiley-VCH |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. Yeramian, Andrée Martin, Lorena Arpa, Luis Bertran, Joan, 1964- Soler Prat, Concepció McLeod, Carol Modolell, Manuel Palacín Prieto, Manuel Lloberas Cavero, Jorge Celada Cotarelo, Antonio Macròfags Proliferació cel·lular Macrophages Cell proliferation In murine macrophages, as a result of arginine catabolism during activation, citruline isproduced under the effect of IFN-c and LPS, and ornithine and polyamines by IL-4 and IL-10. For proliferation, arginine is required from the extracellular medium and is used for protein synthesis. During activation, most arginine (>95% in 6 h) was metabolized, while under proliferation only half was incorporated into proteins. Under basal conditions, this amino acid was preferentially transported by y+L activity. During activation, arginine transport increased drastically (4–5-fold) through y+ cationic amino acid transporter (CAT) activity. By contrast, M-CSF induced only a modest increase in uptake (0.5-fold). The increase in arginine transport during activation, but not proliferation, was mediated by the SLC7A2/Cat2 gene. SLC7A1/Cat1 is constitutively expressed, and is not modified by proliferating or activating agents.M-CSF-dependent proliferation was not affected in the macrophages of SLC7A2 knockout mice; however, these cells showed a drastic reduction in the production of citruline or ornithine and polyamines during activation. The data show that a large increase in a specific transport system (CAT2) is necessary for activation-induced arginine metabolism, while arginine is in excess for the requirements of proliferation and a modest increase in transport occurs. Wiley-VCH https://hdl.handle.net/2445/206968 |
| spellingShingle | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. Yeramian, Andrée Macròfags Proliferació cel·lular Macrophages Cell proliferation |
| status_str | acceptedVersion |
| title | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. |
| title_full | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. |
| title_fullStr | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. |
| title_full_unstemmed | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. |
| title_short | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. |
| title_sort | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. |
| topic | Macròfags Proliferació cel·lular Macrophages Cell proliferation |
| topic_facet | Macròfags Proliferació cel·lular Macrophages Cell proliferation |
| url | https://hdl.handle.net/2445/206968 |