Efeitos da utilização intraperitoneal de Ropivacaína 0,2% na sobrevida de ratos submetidos à peritonite fecal
OBJECTIVE: To evaluate the survival, the histopathological features in lungs, peritoneum, liver and to asses the cytokine serum levels of IL-6 and TNF- in rats subject to fecal peritonitis and treated with peritoneal lavage with 0,2% ropivacaíne by peritoneal lavage. METHODS: We subject 36 Wistar ra...
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| Format: | Doctoral thesis |
| Status: | Published version |
| Publication date: | 2012 |
| Country: | Brasil |
| Institution: | UFMG |
| Repository: | Repositório Institucional da UFMG |
| Language: | Portuguese |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/BUOS-95GQGR |
| Online Access: | http://hdl.handle.net/1843/BUOS-95GQGR |
| Access Level: | Open access |
| Keyword: | TNF- Ratos Histopatologia Peritonite Ropivacaína Sobrevida IL-6 Lavagem peritoneal Peritonio/anatomia & histologia Modelos animais de doenças Resultado de tratamento Fígado/anatomia & histologia Ratos Wistar Citocinas Anestésicos locais/administração & dosagem Avaliação pré-clínica de medicamentos Pulmão/anatomia & histologia |
| Summary: | OBJECTIVE: To evaluate the survival, the histopathological features in lungs, peritoneum, liver and to asses the cytokine serum levels of IL-6 and TNF- in rats subject to fecal peritonitis and treated with peritoneal lavage with 0,2% ropivacaíne by peritoneal lavage. METHODS: We subject 36 Wistar rats (280-320 g) divided in two groups A (n=20) and B (n=16) to laparotomy six hours after the induction of fecal peritonitis with autogenous stool and subseqently divided the rats randomly into four subgroups of five (Group A) and four (Group B) animals: I- Control , no treatment; II- Drying of the abdominal cavity; III- Lavage of abdominal cavity with 3 mL of 0,9% normal saline and drying; IV- Lavage of the abdominal cavity with 3 mL of 0,2% ropivacaíne and drying. In the group A the animals of that died underwent necropsy, and the surviving ones were subjected to euthanasia on the 11th day post-surgery. Fragments of liver, lungs and peritoneum were removed for histological evaluation. In the group B six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized. RESULTS: The animals of group A that received peritoneal lavage (subgroups III and IV) showed greater survival than the drying and control subgroups. Lavage with ropivacaíne prevent dead and maintained the architecture of the lung, peritoneum and liver without any important histological alterations. In the group B the lavage with ropivacaíne resulted in smaller TNF- levels compared with those observed in other treatment groups (p<0,05). Regarding IL-6 ropivacaíne group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p>0,05) between subgroups III and IV. CONCLUSION: Peritoneal lavage with 0,2% ropivacaíne demonstrates a reduction the mortality and histopathological alterations related to inflammatory response and was show to reduce plasma levels of IL-6 and TNF-. |
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