A colorimetric method for the assay of ADP-glucose pyrophosphorylase
The purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples...
| Autores: | ; ; ; ; |
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| Tipo de documento: | Artigo |
| Estado: | Published version |
| Data de publicação: | 2006 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | Inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/85780 |
| Acesso em linha: | http://hdl.handle.net/11336/85780 |
| Nível de acesso: | Acesso aberto |
| Palavra-chave: | ADP-GLUCOSE PYROPHOSPHORYLASE https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Resumo: | The purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples. The method quantifies inorganic orthophosphate released from the specific hydrolysis of the enzyme activity products. For ADPGlc synthesis, the method measures Pi after hydrolysis of PPi by inorganic pyrophosphatase. Pyrophosphorolysis is assayed by determining Pi derived from CF1-ATPase-mediated hydrolysis of ATP. Pi dosage is performed by the technique based in the formation of a phosphomolybdate?Malachite Green complex [14,15]. We optimized the procedure to a microscale grade, reaching convenient sensitivity and the possibility of automation, by using a microplate (multiwell) absorbance reader |
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