Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats

Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient feto–placental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placen...

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Autores: White, Verónica; Capobianco, Evangelina Lorena; Higa, Romina Daniela; Martinez, Nora Alicia; Sosa, María; Jawerbaum, Alicia Sandra
Tipo de documento: Artículo
Estado:Published version
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Lenguaje:Inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/17253
Acceso en línea:http://hdl.handle.net/11336/17253
Nivel de acceso:Acceso abierto
Palabra clave:Placenta
Sod
Peroxynitrite
Nitrotyrosine
Cu/Zn
Diabetes
Rat
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Sumario:Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient feto–placental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placenta from diabetic rats at mid-gestation. Protein nitration was evaluated by EIA, Cu/Zn SOD activity by inhibition of the epinephrine auto-oxidation, Cu/Zn SOD expression by western blot and specific nitration by immunoprecipitation. This study found higher levels of protein nitration (p < 0.001), diminished Cu/Zn SOD activity and enhanced protein expression (p < 0.01) in placentas from diabetic rats. Placental Cu/Zn SOD activity was inhibited by peroxynitrite (p < 0.01). Besides, nitration of Cu/Zn SOD was elevated in placentas from diabetic rats (p < 0.01). These results show that rat Cu/Zn SOD can be nitrated, a modification that could lead to the depressed activity of this enzyme found in placentas from diabetic rats.

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