Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cel...
| Autores: | ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artigo |
| Estado: | Published version |
| Data de publicação: | 2011 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | Inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/115297 |
| Acesso em linha: | http://hdl.handle.net/11336/115297 |
| Nível de acesso: | Acesso aberto |
| Palavra-chave: | osmotic dehydration cell compartments DSC NMR TEM https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| _version_ | 1878403338896146432 |
|---|---|
| author | Dalla Rosa, Marco |
| author2 | Tylewicz, Urszula Panarese, Valentina Laghi, Luca Pisi, Annamaria Santagapita, Patricio Roman Rocculi, Pietro |
| author2_role | author author author author author author |
| author_browse | Dalla Rosa, Marco Laghi, Luca Panarese, Valentina Pisi, Annamaria Rocculi, Pietro Santagapita, Patricio Roman Tylewicz, Urszula |
| author_facet | Dalla Rosa, Marco Tylewicz, Urszula Panarese, Valentina Laghi, Luca Pisi, Annamaria Santagapita, Patricio Roman Rocculi, Pietro |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cellular compartment modifications of the kiwifruit outer pericarp tissue during osmotic dehydration treatment (OD). Two kiwifruit species, A. deliciosa and A. chinensis were submitted to OD. OD was performed in a 61.5 % w/v sucrose solution at three different temperatures (25, 35 and 45 °C), with treatment time from 0 to 300 min. Peleg’s model highlighted that the main response differences between the two kiwifruit species occurred during the initial phase of the osmotic treatment. DSC parameters appeared to be sensitive to water and solid exchange between fruits and osmotic solution. LF-NMR proton T2 revealed the consequences of the water-solid exchange on the cell compartments, namely vacuole, cytoplasm plus extracellular space and cell wall. During OD, the reduction of the vacuole proton pool, detected by LF-NMR, suggested a shrinkage of such compartment, confirmed by LM. Cell walls of outer pericarp showed considerable changes in size, structure and stain uptake during OD observed at TEM. The proposed multianalytical approaches should enable better design of combined processing technologies permitting the evaluation of their effects on tissue response. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_6b9a88f7a2e4c14e828eca5006fd91c4 |
| identifier_str_mv | Dalla Rosa, Marco; Tylewicz, Urszula; Panarese, Valentina; Laghi, Luca; Pisi, Annamaria; et al.; Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study; National Society of Processing and Energy in Agriculture; Journal on Processing and Energy in Agriculture; 15; 3; 10-2011; 113-117 1821-4487 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/115297 |
| publishDate | 2011 |
| publishDateSort | 2011 |
| publisher | National Society of Processing and Energy in Agriculture |
| publisherStr | National Society of Processing and Energy in Agriculture |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study Uticaj osmotske dehidracije na kivi: rezultati multianalitičkog pristupa proučavanja strukture Dalla Rosa, Marco Tylewicz, Urszula Panarese, Valentina Laghi, Luca Pisi, Annamaria Santagapita, Patricio Roman Rocculi, Pietro osmotic dehydration cell compartments DSC NMR TEM https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cellular compartment modifications of the kiwifruit outer pericarp tissue during osmotic dehydration treatment (OD). Two kiwifruit species, A. deliciosa and A. chinensis were submitted to OD. OD was performed in a 61.5 % w/v sucrose solution at three different temperatures (25, 35 and 45 °C), with treatment time from 0 to 300 min. Peleg’s model highlighted that the main response differences between the two kiwifruit species occurred during the initial phase of the osmotic treatment. DSC parameters appeared to be sensitive to water and solid exchange between fruits and osmotic solution. LF-NMR proton T2 revealed the consequences of the water-solid exchange on the cell compartments, namely vacuole, cytoplasm plus extracellular space and cell wall. During OD, the reduction of the vacuole proton pool, detected by LF-NMR, suggested a shrinkage of such compartment, confirmed by LM. Cell walls of outer pericarp showed considerable changes in size, structure and stain uptake during OD observed at TEM. The proposed multianalytical approaches should enable better design of combined processing technologies permitting the evaluation of their effects on tissue response. U ovom radu su prikazani rezultati poređenja različitih analitičkih tehnika (Diferencijalna skeniranja Kalorimetrija – DSC, Niska Polje nuklearna magnetna rezonanca - LF-NMR, svetlost mikroskopije - LM i Prenos elektronsku mikroskopiju - TEM), u cilju određivanja prenosa mase, statusa vode i modifikacije ćelija tkiva perikarpa kivija tokom osmotske dehidratacije tretmana (OD). Dve sorte kivija su bile izložene osmotskom tretmanu, A. deliciosa i A. chinensis. Osmotsko sušenje obavljeno je u 61,5% rastvoru saharoze na tri različite temperature (25, 35 i 45°C), sa vremenom trajanja sušenja od 0 do 300 minuta. Pelegovim modelom naglašene su glavne razlike između dve sorte kivija koje se događaju tokom početne faze osmotskog tretmana. DSC parametri osetljivi su na izmenu vode i čvrste materije između voća i osmotskog rastvora. LF-NMR-om otkrivene su posledice razmene vode i čvrste materije na ćeliju, odnosno vakuole, citoplazmu sa vanćelijskim prostorom i ćelijski zid. Tokom OD, smanjenje vakuole protona bazena, otkrivena je LF-NMR, predložio skupljanja takvog odeljka, potvrđeno LM. Ćelijske zidove spoljne perikarpa su pokazali značajne promene u veličini, strukturi i mrlja uzimanja u toku OD posmatrano na sistem. Predloženi multianalitical pristupi treba da omogući bolji dizajn u kombinaciji obrade tehnologija dozvoljava procenu njihovog uticaja na tkivo odgovor. Fil: Dalla Rosa, Marco. Università di Bologna; Italia Fil: Tylewicz, Urszula. Università di Bologna; Italia Fil: Panarese, Valentina. Università di Bologna; Italia Fil: Laghi, Luca. Università di Bologna; Italia Fil: Pisi, Annamaria. Università di Bologna; Italia Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rocculi, Pietro. Università di Bologna; Italia National Society of Processing and Energy in Agriculture http://hdl.handle.net/11336/115297 Dalla Rosa, Marco; Tylewicz, Urszula; Panarese, Valentina; Laghi, Luca; Pisi, Annamaria; et al.; Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study; National Society of Processing and Energy in Agriculture; Journal on Processing and Energy in Agriculture; 15; 3; 10-2011; 113-117 1821-4487 CONICET Digital CONICET |
| spellingShingle | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study Dalla Rosa, Marco osmotic dehydration cell compartments DSC NMR TEM https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study |
| title_full | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study |
| title_fullStr | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study |
| title_full_unstemmed | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study |
| title_short | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study |
| title_sort | Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study |
| topic | osmotic dehydration cell compartments DSC NMR TEM https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| topic_facet | osmotic dehydration cell compartments DSC NMR TEM https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/115297 |